In vitro Regeneration and Genetically Transformed Culture of Artemisia diffusa
- سال انتشار: 1400
- محل انتشار: فصلنامه گزارش های زیست فناوری کاربردی، دوره: 8، شماره: 3
- کد COI اختصاصی: JR_JABR-8-3_013
- زبان مقاله: انگلیسی
- تعداد مشاهده: 380
نویسندگان
Zanjan Pharmaceutical Biotechnology Research Center, Zanjan University of Medical Sciences, Zanjan, Iran
Zanjan Pharmaceutical Biotechnology Research Center, Zanjan University of Medical Sciences, Zanjan, Iran
School of Computer, Mathematical & Natural Sciences, Morgan State University, Baltimore, MD, USA, ۲۱۲۵۱
Zanjan Pharmaceutical Biotechnology Research Center, Zanjan University of Medical Sciences, Zanjan, Iran
Zanjan Pharmaceutical Biotechnology Research Center, Zanjan University of Medical Sciences, Zanjan, Iran
چکیده
Introduction: The present study has introduced a simple and rapid tissue culture system aimed at in vitro regeneration of Artemisia diffusa and in vitro artemisinin production in its genetically transformed culture.Materials and Methods: An in vitro regeneration of A. diffusa was developed using different combinations of plant growth regulators including Naphthalene Acetic Acid (NAA), Indole-۳-Acetic Acid (IAA), Thidiazuron (TDZ) and Benzyl Adenine (BA). Also, an efficient genetically transformed root induction system for A. diffusa was developed through Agrobacterium rhizogenes- mediated transformation using four bacterial strains, A۴, ATCC۱۵۸۳۴, MSU۴۴۰, and MAFF-۰۲-۱۰۲۶۶. The stem and leaf of one month old sterile plants of A. diffusa were used as explants. Molecular analysis of transformed root lines was confirmed by PCR using primers specific for the rolB gene.Results: The highest regeneration occurrence was obtained using MS medium containing ۰.۵ mg/L TDZ and ۰.۱ mg/L IAA (۷۵%). Root induction was obtained on MS medium supplemented with ۰.۵ mg/L IBA. The results showed a significant increase in transformation frequency when the strain MSU۴۴۰ was used (۸۰.۷%). Approximately ۰.۰۵ % artemisinin was detected by High-performance liquid chromatography (HPLC) analysis in root cultures. To the best of our knowledge, this is the first report of A. diffusa in vitro organogenesis and transformation.Conclusions: This study describes an efficient protocol for hairy roots culture of A. diffusa which can be used for scaling up the plant active phytochemicals or for genetic manipulations of the plant.کلیدواژه ها
Artemisia Diffusa, Artemisinin, Genetically Transformed Culture, In vitro Regenerationاطلاعات بیشتر در مورد COI
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