Regeneration of Sciatic Nerve Injury by Polyglycolic Acid/Collagen/Bioglass Conduit

سال انتشار: 1400
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 266

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شناسه ملی سند علمی:

JR_JABR-8-3_010

تاریخ نمایه سازی: 15 دی 1400

چکیده مقاله:

Introduction: The repair process of severed peripheral nerves is conducted by the bridging of the regenerating neural fibers across a gap in the case of the existence of an appropriate route (space) between the proximal and distal severed stumps. The current study aimed to improve the transected sciatic nerve of rats with a ۱۰ mm gap by means of applying electrospun conduits composed of polymer nanocomposites of polyglycolic acid (PGA), collagen, and nanobioglass (NBG). Then, the efficacy of the designed conduits (PGA/collagen/NBG, PGA/collagen, and PGA alone) was histologically and electrophysiologically compared with autograft nerves to determine whether these conduits have superiority over the autograft procedures in the process of nerve regeneration.Materials and Methods: In this experiment, ۵۰ healthy adult male Wistar rats underwent sciatic nerve transection. After four, eight, and ۱۲ weeks of the surgical procedures, the therapeutic effects of conduits on sensory and motor recovery of transected nerves were evaluated.Results: The analysis of the functions of motor and sensory nerves showed marked improvement in rats treated with PGA/collagen/NBG conduit. Also, histological staining and immunohistochemical assessment of the expression of NF۲۰۰, S۱۰۰, and CD۳۱ proteins revealed newly-formed nerve fibers with micro blood vessels at the proximity of regenerated nerve fibers.Conclusions: It seems that due to the high surface area of electrospun nerve conduits to adhere the cells, the application of these compounds would be beneficial in clinical practice in the future. The results suggest that PGA/Collagen/NBG nanofibrous conduit possesses the highest capability in increasing nerve regeneration following nerve transection in murine models.

نویسندگان

Navid Dehnavi

Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

Kazem Parivar

Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

Vahabodin Goodarzi

Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

Ali Salimi

Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

Kourosh Mansoori

Neuromusculoskletal Research Centre Firozgar Hospital, Iran University of Medical Science, Tehran, Iran

Mohammad Reza Nourani

Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran