Association between single nucleotide polymorphisms rs۷۲۵۲۵۵۳۲, rs۴۵۵۹۶۷۳۸, rs۱۴۸۷۵۹۲۱۶, rs۱۸۸۱۳۳۹۳۶, and rs۱۱۴۶۲۷۱۲۲ of APOA۵ gene in children and adolescents with metabolic syndrome

Background and aims: The APOA۵ gene is one of the genes involved in metabolic syndrome (MetS), as a constellation of several cardiovasculardisease (CVD) risk factors. The present study evaluated the possible associations between five single nucleotide polymorphisms (SNPs) inthe microRNA target site (miR-TS-SNPs) of the APOA۵ gene with MetS.Methods: This case-control study included ۵۷ MetS cases, along with ۵۹ normal children and adolescents aged ۹-۱۸ years. All miR-TSSNPsrs۱۸۸۱۳۳۹۳۶, rs۷۲۵۲۵۵۳۲, rs۴۵۵۹۶۷۳۸, rs۱۴۸۷۵۹۲۱۶, and rs۱۱۴۶۲۷۱۲۲ were genotyped by polymerase chain reaction-sequencing.Independent t-test, as well as the chi-square test and logistic regression analysis was used to determine the association of SNPs with MetSrisk and its clinical components.Results: The mean (SD) age of MetS participants and controls was ۱۲.۳۵ (۰.۲۵) and ۱۳.۳۹ (۰.۳۸) years, respectively. Although no nucleotidechanges were present in rs۱۸۸۱۳۳۹۳۶, rs۴۵۵۹۶۷۳۸, rs۱۴۸۷۵۹۲۱۶, and rs۱۱۴۶۲۷۱۲۲, a greater frequency of A insertion was detected inrs۷۲۵۲۵۵۳۲ in MetS cases compared with the control group (P = ۰.۰۱۲). This variant showed a significant difference in triglycerides (TG)and high-density lipoprotein cholesterol (HDL) levels between different genotype groups (P<۰.۰۰۰۱ and P = ۰.۰۵, respectively) in controls.Furthermore, AA insertion genotype was correlated with an increased risk of MetS (Odds ratio [۹۵% CI] = ۸.۱۲ [۰.۹۶۶-۶۸.۲۷], P = ۰.۰۵).Conclusion: This study was the first to investigate the association between rs۱۸۸۱۳۳۹۳۶, rs۴۵۵۹۶۷۳۸, rs۱۴۸۷۵۹۲۱۶, rs۷۶۴۶۳۵۲۴, andrs۷۲۵۲۵۵۳۲ variants of the APOA۵ gene and MetS. Our findings reveal that rs۷۲۵۲۵۵۳۲ might have an impact on TG, HDL levels, andthe risk of MetS.

کلیدواژه ها:

Metabolic syndrome ، APOA۵ ، Single nucleotide polymorphism ، miRNA