Tahereh Agha Motallebi Khobani - Microbiology Department, Faculty of biological sciences, Alzahra University, Tehran, Iran
Bahareh Attaran - Microbiology Department, Faculty of biological sciences, Alzahra University, Tehran, Iran
Mohammad Reza soudi - Microbiology Department, Faculty of biological sciences, Alzahra University, Tehran, Iran
Tayebeh Fooladi - Microbiology Department, Faculty of biological sciences, Alzahra University, Tehran, Iran

Ganoderma sp. is a medicinal mushroom producing biologically active substances such as sterols, proteins, polysaccharides, and triterpenoids. Helicobacter pylori strains have become increasingly resistant to antibiotics in recent years. In this article, the effect of Ganoderma fungal extract to inhibit these bacteria has been evaluated. In this study, inhibition zone and minimum inhibitory concentration (MIC) values of extracts prepared from the fruiting bodies of Ganoderma lucidum were determined against H. pylori using seven clinical isolates via an in vitro disc diffusion and agar dilution assay according to the CLSI standard. Amoxicillin was used as control. The crude Methanolic extracts of G. lucidum (۱۰ mg/ml) inhibited the growth of H. pylori in vitro, with the inhibition zone diameter ۲۴ to ۴۰ mm. The inhibition of H. pylori was observed through an agar dilution test with minimal inhibition concentration (MIC) values from ۸۵۰ μg/mL to ۱۰۰۰ μg/mL. This finding indicates that the compounds in Ganoderma species are active against Helicobacter pylori in vitro. Due to humans' growing reluctancy from synthetic drugs and tendency toward natural bioactive remedies, fungi can be a suitable source to meet this need. However, in vivo assessment should be done.

Ganoderma lucidum, Helicobacter pylori, Antimicrobial properties, Antibiotic resistance