Assessment of IgM/IgG Antibody Detection in Comparison with RT-PCR Technique for Diagnostic Purposes in Patients with COVID-۱۹
- سال انتشار: 1400
- محل انتشار: فصلنامه میکروب شناسی پزشکی ایران، دوره: 15، شماره: 5
- کد COI اختصاصی: JR_IJMM-15-5_006
- زبان مقاله: انگلیسی
- تعداد مشاهده: 215
نویسندگان
Department of Clinical Biochemistry, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Department of Clinical Biochemistry, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Abadan university of medical sciences, Abadan, Iran
Abadan University of Medical Sciences, Abadan, Iran
Abadan University of Medical Sciences, Abadan, Iran
Department of Clinical Biochemistry, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
چکیده
Background and Aim: The diagnosis of COVID-۱۹ is an essential step toward controlling the pandemic. For this purpose, a series of laboratory methods have been developed. This study evaluated the efficiency of the ELISA method for COVID-۱۹ IgM and IgG detection. Materials and Methods: In this case-control study, ۴۶ blood samples from PCR positive COVID-۱۹ patients and ۴۹ samples from PCR negative COVID-۱۹ subjects were collected. Subsequently, the presence of IgM and IgG in all blood samples was assessed using the Pishtaz Teb ELISA kit. Results: The data demonstrated that among ۴۹ PCR negative, ۴۰ (۸۱.۶%) were IgM negative and ۹ (۱۹.۱%) were IgM positive. Besides, out of the ۴۹ PCR-negative patients, ۴۲ (۸۵.۷%) and ۷ (۱۴.۳%) were IgG negative and positive, respectively. In ۴۶ PCR-positive individuals, ۴۰ (۸۷%) were IgM negative, while ۶ (۱۳ %) were IgM positive. Of the ۴۶ PCR-positive patients, ۲۴ (۵۲.۲%) were IgG negative, and ۲۲(۴۷.۸%) were IgG positive. Conclusion: Our results showed that detection of SARS-CoV-۲ IgG and IgM using Pishtaz Teb ELISA kit is not enough for COVID diagnosis, but it can serve as a diagnostic RNA supplement to confirm infection with SARS-CoV-۲ in approved clinics and other scientific communities, owing to its easy, rapid, and inexpensive availability.کلیدواژه ها
Antibody, COVID-۱۹, IgM/IgG, Patients, Antibody, COVID-۱۹, IgM/IgG, Patientsاطلاعات بیشتر در مورد COI
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