Somatic embryogenesis and bulblet production in <I>Narcissus papyraceus</I> cv. Shirazi: effect of plant growth regulators, light intensity, sucrose concentration, methyl jasmonate and anti-gibberellins

Generally, narcissus is propagated through vegetative methods, but due to their low yield their are not economical. This study was conducted to develop a suitable method for the production of somatic embryos and bulblets in Narcissus papyraceus cv. Shirazi. Bulb scales were placed on the MS medium supplemented with ۲,۴-D and BAP each at ۰.۰, ۰.۲۵, ۰.۵ or ۱ mg/l concentrations to form somatic embryos (SEs). For proliferation, the bulblets were cut in small segments and placed on either MS medium supplemented with sucrose at ۳۰, ۶۰ or ۹۰ g/l, kept in dark or light intensities of ۵۴ or ۱۰۸ μmol m-۲ s-۱ or the MS medium including Methyl jasmonate (Me JA), growth retardants Paclobutrazol or Cycocel (PBZ or CCC) each at ۰.۰, ۰.۵, ۱.۰ or ۲.۰ mg/l. The highest SE percentage (۷۵ ± ۹.۰) was obtained when ۱.۰ mg/l ۲,۴-D and ۰.۵ mg/l BAP were incorporated into the medium. Using sucrose at ۶۰ g/l under a light intensity of ۵۴ μmol m-۲ s-۱ or utilizing ۱.۰ mg/l PBZ produced the greatest bulblet number (۴.۴۵ ± ۰.۵۱ and ۵.۰ ± ۰.۲۵, respectively). None of the scales produced bulblets in dark at any sucrose concentration.

کلیدواژه ها:

Bulblet production ، Growth retardants ، Light intensity ، Methyl jasmonate ، Somatic embryogenesis ، Sucrose