Elaheh Ouladsahebmadarek - Women’s Reproductive Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Arash Khaki - Department of Pathology, Tabriz Branch, Islamic Azad University, Tabriz, Iran
Sharareh Khanahmadi - Women’s Reproductive Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Hamidreza Ahmadi Ashtiani - Department of Biochemistry, Islamic Azad University, Pharmaceutical, Tehran, Iran
Pooya Paknejad - Women’s Reproductive Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Mohammad Reza Ayubi - Department of Pathology, Tabriz Branch, Islamic Azad University, Tabriz, Iran

Objective(s): PCOS (polycystic ovary syndrome) produces symptoms in approximately ۵% to ۱۰% of women of reproductive age (۱۲–۴۵ years old). It is thought to be one of the leading causes of female subfertility. This study aimed to confirm the role of nutrition containing omega-۳ (polyunsaturated fatty acid) on control of experimental PCO induced by estradiol-valerat in rats. Materials and Methods: Wistar female rats (n=۴۰) were allocated into control (n=۱۰) and test groups (n= ۳۰), test group was subdivided into ۳ groups: G۱, received omega-۳ (۲۴۰ mg/kg/orally/daily); G۲ and G۳ groups were induced PCO by single injection of estradiol-valerate (۱۶ mg/kg/IM). Group ۳ received omega-۳ (۲۴۰ mg/kg/orally/daily) and low carbohydrate feeding for ۶۰ subsequent days; on sixtieth day ۵ ml blood samples and ovarian tissues of all rats in the group were removed and prepared for biochemical and hormonal analysis. Results: Catalase, GPX (Glutathione peroxidase), SOD (Superoxide dismutase) in groups that received omega-۳showed higher levels, but MDA (malondialdehyde) level was significantly decreased (P<۰.۰۵) in comparison with other experimental groups. Ovarian weights in both experimental and control groups were similar (P<۰.۰۵). Level of serum FSH (follicle stimulating hormone) was decreased, but level of testosterone was significantly increased (P<۰.۰۵) in PCO group in comparison with control and omega-۳ groups. Conclusion: Results revealed that administration of omega-۳ plus lower carbohydrate food significantly controlled   PCO syndrome and balanced FSH and testosterone.

Catalaze (CAT), Follicle stimulating hormone (FSH), Glutathione peroxidase (GPX), Omega-۳, Polycystic ovary (PCO), Super oxide dismutase (SOD), Testosterone