Identification of Novel Hypoxia Response Genes in Human Glioma Cell Line A۱۷۲
- سال انتشار: 1392
- محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 16، شماره: 5
- کد COI اختصاصی: JR_IJBMS-16-5_003
- زبان مقاله: انگلیسی
- تعداد مشاهده: 317
نویسندگان
۱Department of Medical Genetics, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
۱Department of Medical Genetics, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Mohammad Hasanzadeh Nazarabadi
۱Department of Medical Genetics, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
۱Department of Medical Genetics, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Department of Clinical Biochemistry, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
۱Department of Medical Genetics, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
۱Department of Medical Genetics, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
۱Department of Medical Genetics, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Ira
۱Department of Medical Genetics, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Department of Clinical Biochemistry, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
۱Department of Medical Genetics, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
چکیده
Objective(s): Hypoxia is a serious challenge for treatment of solid tumors. This condition has been manifested to exert significant therapeutic effects on glioblastoma multiform or (WHO) astrocytoma grade IV. Hypoxia contributes numerous changes in cellular mechanisms such as angiogenesis, metastasis and apoptosis evasion. Furthermore, in molecular level, hypoxia can cause induction of DNA breaks in tumor cells. Identification of mechanisms responsible for these effects can lead to designing more efficient therapeutic strategies against tumor progression which results in improvement of patient prognosis. Materials and Methods: In order to identify more hypoxia regulated genes which may have a role in glioblastoma progression, cDNA-AFLP was optimized as a Differential display method which is able to identify and isolate transcripts with no prior sequence knowledge. Results: Using this method, the current study identified ۱۲۰ Transcription Derived Fragments (TDFs) which were completely differentially regulated in response to hypoxia. By sequence homology searching, the current study could detect ۲۲ completely differentially regulated known genes and two unknown sequence matching with two chromosome contig and four sequence matches with some Expressed Sequence Tags (ESTs). Conclusion: Further characterizing of these genes may help to achieve better understanding of hypoxia mediated phenotype change in tumor cells.کلیدواژه ها
cDNA-AFLP Glioblastoma Hypoxiaاطلاعات بیشتر در مورد COI
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