The role of local renin-angiotensin system on high glucose-induced cell toxicity, apoptosis and reactive oxygen species production in PC۱۲ cells

  • سال انتشار: 1393
  • محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 17، شماره: 8
  • کد COI اختصاصی: JR_IJBMS-17-8_012
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 215
دانلود فایل این مقاله

نویسندگان

Kaveh Shahveisi

Neurocognitive Research Center and Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran ۲ Sleep Disorders Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

Seyed Hadi Mousavi

Pharmacological Research Center of Medicinal Plants and Department of Pharmacology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Mahmoud Hosseini

Neurocognitive Research Center and Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Abolfazl Khajavi Rad

Applied Physiology Research Center and Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Seyed Amir Jalali

Immunogenetic and Cell Culture Department, Immunology Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran North Khorasan University of Medical Sciences, Bojnurd, Iran

Ziba Rajaei

Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Hamid Reza Sadeghnia

Neurocognitive Research Center and Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Mousa-Al-Reza Hadjzadeh

Applied Physiology Research Center and Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

چکیده

Objective(s): Hyperglycemia, oxidative stress and apoptosis have key roles in pathogenesis of diabetic neuropathy. There are local renin-angiotensin systems (RASs) in different tissues such as neural tissue. Local RASs are involved in physiological and pathophysiological processes such as inflammation, proliferation and apoptosis. This study aimed to investigate the role of local renin-angiotensin system on high glucose-induced cell toxicity, apoptosis and reactive oxygen species (ROS) production in PC۱۲ cells, as a cell model of diabetic neuropathy. Materials and Methods: PC۱۲ cells were exposed to a high glucose concentration (۲۷ mg/ml), captopril (ACE inhibitor), telmisartan and losartan (AT۱ antagonists), and also PD۱۲۳۳۱۹ (AT۲ antagonist) were administered before and after induction of high glucose toxicity. Then cell viability was assessed by MTT assay and apoptotic cells and intracellular ROS production were detected by annexin V-propidium iodide and DCFDA, respectively, using flow cytometry. Results: High glucose concentration decreased cell viability, and increased apoptotic cells. Intracellular ROS production was also increased. In PC۱۲ cells pretreatment and treatment by the drugs showed a significant improvement in cell viability and reduced apoptosis in captopril, telmisartan and PD۱۲۳۳۱۹ but only captopril and telmisartan were able to reduce ROS production. Losrtan significantly lowered ROS but didn’t show any improvements in cell viability and apoptotic cells. Conclusion:  The results of the present study showed that RAS inhibitors reduced cell toxicity and apoptosis and ROS production was induced by high glucose. It may be suggested that local RAS has a role in high glucose toxicity.

کلیدواژه ها

Apoptosis, High glucose toxicity, Oxidative stress, PC۱۲, Renin-angiotensin system

اطلاعات بیشتر در مورد COI

COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.

کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.