The effect of BSO-induced oxidative stress on histologic feature of testis: testosterone secretion and semen parameters in mice
- سال انتشار: 1393
- محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 17، شماره: 8
- کد COI اختصاصی: JR_IJBMS-17-8_011
- زبان مقاله: انگلیسی
- تعداد مشاهده: 165
نویسندگان
Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Iran
Neuroscience Research Center, Faculty of Medicine, Tabriz University of Medical Sciences, Iran
Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Iran
Biochemical Department, Faculty of Medicine, Medical Sciences University of Tabriz, Iran
Department of Gynecology, Faculty of Medicine, Tabriz university of Medical Sciences, Iran
Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Iran
چکیده
Objective(s): Buthionine sulfoximine (BSO) inhibits synthesis of glutathione as the main intracellular antioxidant. The aim of the present study is to investigate the effect of BSO-induced oxidative stress on histological structure of testis, testosterone secretion and semen parameters. Materials and Methods: Thirty male BALB/c mice were divided into ۳ groups. In control group, the mice did not receive any chemical. In the experimental group, the mice received ۲ mmol/kg BSO for ۳۵ days. In the sham group, the mice received the solvent of BSO (۰.۹% saline). After the treatment, the mice were sacrificed. Their testes were fixed in Buein’s fixative, embedded in paraffin and prepared for histological studies. To assess semen parameters, the sperms were collected from cauda epididymis. Blood samples were used for determination of super oxide dismutase (SOD), malondialdehyde (MDA), glutathione peroxidase (GPX), glutathione (GSH), catalase (CAT) and the serum testosterone level. The data analyzed using ANOVA and Dunnett's tests and SPSS software, version۱۱.۵. P- values at ۰.۰۵ level considered significant. Results: Data showed that in experimental group in comparison to control group; the concentration of CAT, GPX, SOD,GSH and the total level of testosterone is reduced while MDA level is increased significantly. The number of sperms with progressive motility were decreased (P< ۰.۰۰۱) but sperms with abnormal morphology were increased (P< ۰.۰۰۱). Histological studies revealed that the values for tubal differentiation index and spermatogenic index in experimental group were reduced (P< ۰.۰۰۱). Conclusion: It is concluded that exposure to oxidative stress induced by BSO could affect testicular structure and semen parameters.کلیدواژه ها
Buthionine sulfoximine, Glutathione, Oxidative Stress Markers, Testisاطلاعات بیشتر در مورد COI
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