Effect of Nigella sativa on ischemia-reperfusion induced rat kidney damage

  • سال انتشار: 1393
  • محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 17، شماره: 12
  • کد COI اختصاصی: JR_IJBMS-17-12_008
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 277
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نویسندگان

Shahrzad Havakhah

Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Hamid R Sadeghnia

Pharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Mosa-Al-Reza Hajzadeh

Neurocognitive Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Nama Mohammadian Roshan

Department of Pathology, Ghaem Hospital, Mashhad University of Medical Sciences, Mashhad, Iran

Somayeh Shafiee

Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Hossein Hosseinzadeh

Pharmaceutical Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

Narges Mohareri

Department of Pharmacodynamy and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

Abolfazl Khajavi Rad

Neuroinflammation Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

چکیده

Objective(s):There are a few previously reported studies about the effect of Nigella sativa oil on renal ischemia-reperfusion injury (IRI). The aim of the present study was to test the hypothesis whether pre- or post-treatment with N. sativa hydroalcoholic extract (NSE) would reduce tissue injury and oxidative damages in a clinically relevant rat model of renal IRI.    Materials and Methods: IRI was induced by clamping of bilateral renal arteries for ۴۰ min fallowed by reperfusion for ۱۸۰ min. NSE was prepared in a Soxhlet extractor and administrated with doses of ۱۵۰ mg/kg or ۳۰۰ mg/kg at ۱ hr before ischemia induction (P-۱۵۰ and ۳۰۰) or at the beginning of reperfusion phase (T-۱۵۰ and ۳۰۰), via jugular catheter intravenously. The kidneys were then removed and subjected to biochemical analysis, comet assay or histopathological examination. Results: The kidneys of untreated IRI rats had a higher histopathological score (P< ۰.۰۰۱), while in P-۱۵۰, as well as T-۱۵۰ and T-۳۰۰ groups tubular lesions significantly decreased (P< ۰.۰۰۱). Pre- and post-treatment with NSE also resulted in a significant decrease in malondialdehyde (MDA) level (P< ۰.۰۰۱) and DNA damage (P< ۰.۰۰۱) that were increased by renal I/R injury. NSE treatment also significantly restore (P< ۰.۰۱) the decrease in renal thiol content caused by IRI. Conclusion: The present study shows N. sativa extract has marked protective action against renal IRI, which may be partly due to its antioxidant effects.

کلیدواژه ها

Comet assay, Ischemia-reperfusion injury (IRI), Kidney, Nigella Sativa, Oxidative stress

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