Estrogen provides neuroprotection against brain edema and blood brain barrier disruption through both estrogen receptors α and β following traumatic brain injury
- سال انتشار: 1394
- محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 18، شماره: 2
- کد COI اختصاصی: JR_IJBMS-18-2_005
- زبان مقاله: انگلیسی
- تعداد مشاهده: 120
نویسندگان
Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran
Physiology Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran
Department of Physiology, Kerman University of Medical Sciences, Kerman, Iran
Physiology Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran
چکیده
Objective(s):Estrogen (E۲) has neuroprotective effects on blood-brain-barrier (BBB) after traumatic brain injury (TBI). In order to investigate the roles of estrogen receptors (ERs) in these effects, ER-α antagonist (MPP) and, ER-β antagonist (PHTPP), or non-selective estrogen receptors antagonist (ICI ۱۸۲۷۸۰) were administered. Materials and Methods: Ovariectomized rats were divided into ۱۰ groups, as follows: Sham, TBI, E۲, oil, MPP+E۲, PHTPP+E۲, MPP+PHTPP+E۲, ICI+E۲, MPP, and DMSO. E۲ (۳۳.۳ µg/Kg) or oil were administered ۳۰ min after TBI. ۱ dose (۱۵۰ µg/Kg) of each of MPP, PHTPP, and (۴ mg/kg) ICI۱۸۲۷۸۰ was injected two times, ۲۴ hr apart, before TBI and estrogen treatment. BBB disruption (Evans blue content) and brain edema (brain water content) evaluated ۵ hr and ۲۴ hr after the TBI were evaluated, respectively. Results: The results showed that E۲ reduced brain edema after TBI compared to vehicle (P< ۰.۰۱). The brain edema in the MPP+E۲ and PHTPP+E۲ groups decreased compared to the vehicle (P< ۰.۰۰۱). There was no significant difference in MPP+PHTPP+E۲ and ICI+E۲ compared to TBI. This parameter in MPP was similar to vehicle. Evans blue content in E۲ group was lower than vehicle (P< ۰.۰۵).The inhibitory effect of E۲ on Evans blue was not reduced by MPP+E۲ and PHTPP+E۲ groups, but decreased by treatment with MPP+PHTPP or ICI. MPP had no effect on Evans blue content. Conclusion: A combined administration of MPP and PHTPP or ICI inhibited the E۲-induced decrease in brain edema and BBB disruption; this may suggest that these effects were mediated via both receptors.کلیدواژه ها
Blood-brain-barrier, Brain edema, ERα antagonist, ERβ antagonist, ICI۱۸۲۷۸۰, Traumatic brain injuryاطلاعات بیشتر در مورد COI
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