Alpha-lipoic acid loaded in chitosan conduit enhances sciatic nerve regeneration in rat
- سال انتشار: 1394
- محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 18، شماره: 3
- کد COI اختصاصی: JR_IJBMS-18-3_003
- زبان مقاله: انگلیسی
- تعداد مشاهده: 319
نویسندگان
Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Stem Cell & Regenerative Medicine Research Group, Department of Clinical Biochemistry, Mashhad University of Medical Sciences, Mashhad, Iran
Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada
Department of Clinical Sciences, Faculty of Veterinary Medicine, Lorestan University, Khoramabad, Iran
Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
چکیده
Objective(s): To investigate the effect of topical administration of alpha-lipoic acid into chitosan conduit on peripheral nerve regeneration using a rat sciatic nerve transection model. Materials and Methods: Forty five Wistar rats were divided into three experimental groups randomly. A ۱۰-mm gap of sciatic nerve was bridged with a chitosan conduit following surgical preparation and anesthesia. In treatment group, the conduit was filled with ۳۰ µl alpha-lipoic acid (۱۰ mg/kg/bw).It was filled with ۳۰ µl phosphate buffered saline solution in control group. In Sham group sciatic nerve was just exposed. Results: The recovery of nerve function was faster in treatment group than in control, at ۴ and ۸ weeks after surgery (P-value< ۰.۰۵). Conduction velocity was better in treatment group than in control group at ۴ and ۱۲ weeks (P-value< ۰.۰۵). Recovery index was higher in treatment group than the control group, ۸ weeks after surgery (P-value < ۰.۰۵). Greater nerve fiber diameter, axon diameter, and myelin sheath thickness were observed in treatment group compared to control group at ۸ and ۱۲ weeks after surgery (P-value< ۰.۰۵). The immunoreactivity of regenerated axons and myelin sheath in treatment group were far more similar to sham group. Conclusion: Alpha-lipoic acid when loaded in a chitosan conduit could improve transected sciatic nerve regeneration in rat.کلیدواژه ها
Alpha-lipoic acid, Chitosan conduit, Rat, Sciatic nerve regenerationاطلاعات بیشتر در مورد COI
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