Sulforaphane production and antioxidant enzyme activity in Lepidium draba seedlings in presence of Yeast extract and silver nitrate
- سال انتشار: 1400
- محل انتشار: مجله گیاهان دارویی، دوره: 2، شماره: 1
- کد COI اختصاصی: JR_ETPHPR-2-1_001
- زبان مقاله: انگلیسی
- تعداد مشاهده: 196
نویسندگان
Kerman Graduate University of Advanced Technology
Department of Biotechnology, Faculty of Science and Modern Technology, Graduate University of Advanced Technology, Kerman, Iran
Department of Biotechnology, Faculty of Science and Modern Technology, Graduate University of Advanced Technology, Kerman, Iran
چکیده
Sulforaphane is produced through a hydrolytic reaction catalyzed by myrosinase from glucoraphanin. It has recently attracted researcher's interest due to its anticancer activities. The present study was conducted to evaluate the effects of various concentrations of yeast extract (YE) and silver ion (Ag+) on sulforaphane production level in Lepidium draba seedlings. Our results revealed that the sulforaphane content is increased in seedlings that treated with the both elicitors, but more significantly by Ag+. All elicitor influences are depend on the elicitor dose and time of elicitation and maximized with YE at ۱ mg/mL for ۸ hours and with Ag+ at ۵۰ µM for ۴ hours treatment. It seems that the promotion of sulforaphane content results in induction of glucoraphanin biosynthesis pathway which in turn is mediated by H۲O۲, as revealed by a significant increase in the activity of H۲O۲ scavenger enzymes. DOR: https://dorl.net/dor/۲۰.۱۰۰۱.۱.۲۳۸۳۳۰۱۷.۲۰۲۱.۲.۱.۱.۱کلیدواژه ها
Antioxidant enzymes, Sulforaphane, yeast extract, Ag+اطلاعات بیشتر در مورد COI
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