Immune reactivity of sera obtained from brucellosis patients and vaccinated-rabbits to a fusion protein from Brucella melitensis
- سال انتشار: 1394
- محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 18، شماره: 4
- کد COI اختصاصی: JR_IJBMS-18-4_006
- زبان مقاله: انگلیسی
- تعداد مشاهده: 387
نویسندگان
Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
چکیده
Objective(s): Brucella spp. are facultative intracellular pathogens which can stay alive and multiply in professional and nonprofessional phagocytes. Immunity against Brucella melitensis involves antigen-specific CD۴+ and CD۸+ T-cells activation and humoral immune responses. Due to negative aspects of live attenuated vaccines, much attention has been focused on finding Brucella-protective antigens to introduce them as potential subunit vaccine candidates. Materials and Methods: A chimeric gene encoding trigger factor (TF), Omp۳۱۴۸-۷۴ and BP۲۶۸۷-۱۱۱ fragments (TOB) from B. melitensis was successfully cloned, expressed in Escherichia coliBL۲۱-DE۳ and purified by Ni-NTA agarose column. Antibodies to recombinant TOB (rTOB) have been investigated in Brucella-infected human sera and a pool serum prepared from B. melitensis-vaccinated rabbits. Results: Our results showed that the immunized rabbit pool serum strongly reacted with rTOB. In addition, antibodies against rTOB were detectable in ۷۶.۵% of sera obtained from infected patients. Conclusion: These findings suggest that rTOB may provide a potential immunogenic candidate which could be considered in future vaccine studies.کلیدواژه ها
Brucella melitensis, ELISA, Fusion protein, Immune reactivity, Vaccineاطلاعات بیشتر در مورد COI
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