The Lcn۲-engineered HEK-۲۹۳ cells show senescence under stressful condition

سال انتشار: 1394
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 222

فایل این مقاله در 6 صفحه با فرمت PDF قابل دریافت می باشد

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:

شناسه ملی سند علمی:

JR_IJBMS-18-5_005

تاریخ نمایه سازی: 3 آبان 1400

چکیده مقاله:

Objective(s): Lipocalin۲ (Lcn۲) gene is highly expressed in response to various types of cellular stresses. The precise role of Lcn۲ has not been fully understood yet. However, it plays a key role in controlling vital cellular processes such as proliferation, apoptosis and metabolism. Recently it was shown that Lcn۲ decreases senescence and increases proliferation of mesenchymal stem cells (MSC) with finite life span under either normal or oxidative stress conditions. However, Lcn۲ effects on immortal cell line with infinite proliferation are not defined completely.  Materials and Material and Methods: HEK-۲۹۳ cells were transfected with recombinant pcDNA۳.۱ containing Lcn۲ fragment (pcDNA۳.۱-Lcn۲). Expression of lipocalin۲ in transfected cells was evaluated by RT-PCR, real time RT-PCR, and ELISA. Different cell groups were treated with H۲O۲ and WST-۱ assay was performed to determine their proliferation rate. Senescence was studied by β-galactosidase and gimsa staining methods as well as evaluation of the expression of senescence-related genes by real time RT-PCR. Results: Lcn۲ increased cell proliferation under normal culture condition, while the proliferation slightly decreased under oxidative stress.  This decrease was further found to be attributed to senescence. Conclusion: Our findings indicated that under harmful conditions, Lcn۲ gene is responsible for the regulation of cell survival through senescence.

نویسندگان

Bahareh Bahmani

Anatomical Sciences Research Center, Kashan University of Medical Sciences, Kashan, Iran

Fatemeh Amiri

Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran

Amaneh Mohammadi Roushandeh

Anatomical Sciences Department, Medicine Faculty, Hamadan University of Medical Sciences, Hamadan, Iran

Marzie Bahadori

Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran

Mozhgan Dehghan Harati

Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran

Mehryar Habibi Roudkenar

Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran

مراجع و منابع این مقاله:

لیست زیر مراجع و منابع استفاده شده در این مقاله را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود مقاله لینک شده اند :
  • ۱. Hayflick L, Moorhead PS. The serial cultivation of human diploid ...
  • ۲. Rodier F, Campisi J. Four Faces of Cellular Senescence. J ...
  • ۳. Ben-Porath I, Weinberg RA. The signals and pathways activating cellular ...
  • ۴. Erenpreisa J, Cragg MS. Three steps to the immortality of ...
  • ۵. Kuilman T, Michaloglou C, Mooi WJ, Peeper DS. The essence ...
  • Santin AD, Zhan F, Bellone S, Palmieri M, Cane S, ...
  • ۷. Missiaglia E, Blaveri E, Terris B, Wang YH, Costello E, ...
  • ۸. Liu Q, Nilsen-Hamilton M. Identification of a new acute phase ...
  • ۹. Nielsen BS, Borregaard N, Bundgaard JR, Timshel S,Sehested M, Kjeldsen ...
  • Mishra J, Mori K, Ma Q, Kelly C, Barasch J, ...
  • Hemdahl AL, Gabrielsen A, Zhu C, Eriksson P, Hedin U, ...
  • Tong Z, Wu X, Ovcharenko D, Zhu J, Chen CS, ...
  • Berger T, Togawa A, Duncan GS, Elia AJ, You-Ten A, ...
  • Rodvold JJ, Mahadevan NR, Zanetti M. Lipocalin۲ in cancer: when ...
  • Mishra J, Dent C, Tarabishi R, Mitsnefes MM, Ma Q, ...
  • Viau A, El Karoui K, Laouari D, Burtin M, Nguyen ...
  • Zhao P, Stephens JM. STAT۱, NF-jB and ERKs play a ...
  • Bahmani B, Roudkenar MH, Halabian R, Jahanian-Najafabadi A, Amiri F, ...
  • Roudkenar MH, Halabian R, Roushandeh AM, Nourani MR, Masroori N, ...
  • Lafferty-Whyte K, Bilsland A, Cairney CJ, Hanley L, Jamieson NB, ...
  • Kasper G, Mao L, Geissler S, Draycheva A, Trippens J, ...
  • Kim JS, Kim EJ, Kim HJ, Yang JY, Hwang GS, ...
  • نمایش کامل مراجع