Prevalence and Molecular Characterization of Plasmid-mediated Extended-Spectrum β-Lactamase Genes (balaTEM, blaCTX and blASHV) Among Urinary Escherichia coli Clinical Isolates in Mashhad, Iran
- سال انتشار: 1391
- محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 15، شماره: 3
- کد COI اختصاصی: JR_IJBMS-15-3_006
- زبان مقاله: انگلیسی
- تعداد مشاهده: 142
نویسندگان
Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran
Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran
چکیده
Objective(s) Extended-spectrum beta-lactamase (ESBL) producing bacteria have an important role in nosocomial infections. Due to the limited availability of information about the molecular epidemiology of ESBL producing bacteria in Mashhad, we decided to investigate about TEM, CTX and SHV ESBLs among urinary Escherichia coli isolates in Mashhad, a city in northeast Iran. Materials and Methods One hundred and eleven clinical isolates of E. coli were diagnosed from hospitalized patients in ۲۰۰۹. After performing antibiogram and phenotypic confirmation test, polymerase chain reaction (PCR) was performed by blaTEM, blaSHV and blaCTX primers and restriction digestion was carried out using PstI and TaqI (Fermentas-Lithuania) for confirmation. Results ESBL producers of E. coli isolates were ۳۳.۳%. Among ۳۷ ESBL-producing isolates, ۳۵ (۹۴.۶%), ۲۱ (۵۶.۸%) and ۵ (۱۳.۵%) were shown to have blaCTX, blaTEM and blaSHV, genes respectively. Co-resistance to non-beta lactam antibiotics was observed more with ESBL producers (P < ۰.۰۵). Conclusion The results showed that the studied ESBL genes are found with high prevalence and among them blaCTX is more widespread in urine E. coli isolates in Mashhad.کلیدواژه ها
Antibiotic resistance, Escherichia coli, Extended-spectrum beta-lactamase, Urinary tract infectionاطلاعات بیشتر در مورد COI
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