Screening and identification of SUMP-proteins in sub-acute treatment with diazinon

  • سال انتشار: 1394
  • محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 18، شماره: 12
  • کد COI اختصاصی: JR_IJBMS-18-12_012
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 241
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نویسندگان

Rezvan Yazdian-Robati

Department of Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

Atena Pourtaji

Department of Pharmacodynamy and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

Marzieh Rashedinia

Department of Toxicology and pharmacology, School of Pharmacy, international branch, Shiraz University of Medical Sciences, Shiraz, Iran

Hossein Hosseinzadeh

Pharmaceutical Research Center, Department of Pharmacology and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

Maryam Ghorbani

Department of Pharmacology and Toxicology, School of Pharmacy, Baqiyatallah University of Medical Sciences, Tehran, Iran

BiBi Marjan Razavi

Targeted Drug Delivery Research Center, Department of Pharmacology and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

Mohammad Ramezani

Pharmaceutical Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

Khalil Abnous

Pharmaceutical Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

چکیده

Objective(s):Small ubiquitin-like modifiers (SUMOs) are a family of ubiquitin-related, proteins that are involved in a wide variety of signaling pathways. SUMOylation, as a vital post translational modification, regulate protein function in manycellular processes. Diazinon (DZN), an organophosphate insecticide, causses oxidative stress and subsequently programmed cell death in different tissues. The aim of this study was to evaluate the role and pattern of SUMO modificationas a defense mechanism against stress oxidative, in the heart tissuesof the DZN treated rats. Materials and Methods: Diazinon (۱۵ mg/kg/day), corn oil (control) were administered via gavageto male Wistar rats for four weeks. SUMO۱ antibody was covalently crosslinked to protein A/G agarose. heart tissue lysate were added to agarosebeads,After isolation of target proteins(SUMO۱- protein)SDS-PAGE gel electrophoresis was performed. Protein bands were identified using MALDI-TOF/TOF and MASCOT). Fold change of (DZN/Ctrl) separated proteins was evaluated using UVband software (UVITEC, UK). Results:Our result showed that subacute exposure to DZN increased SUMOylationoffour key proteins involved in the metabolic process including; Acyl-CoA dehydrogenase, creatine kinase, glyceraldehyde-۳-phosphate dehydrogenase and ATP synthase, in the heart tissue of animals .A probability value of  less than ۰.۰۵ was considered significant (P< ۰.۰۵). Conclusion: It seems that protein SUMOylation provides a safeguard mechanism against DZN Toxicity.

کلیدواژه ها

Diazinon, MALDI-TOF/TOF, SUMOlation, SUMO۱

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