Inactivation of mitogen-activated protein kinase signaling pathway reduces caspase-۱۴ expression in impaired keratinocytes
- سال انتشار: 1395
- محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 19، شماره: 1
- کد COI اختصاصی: JR_IJBMS-19-1_004
- زبان مقاله: انگلیسی
- تعداد مشاهده: 184
نویسندگان
Department of Dermatology, Jinan Central Hospital affiliated to Shandong University, Jinan, Shandong Province, ۲۵۰۰۱۳, China
Department of Endocrinology, Jinan Central Hospital affiliated to Shandong University, Jinan, Shandong Province, ۲۵۰۰۱۳, China
Department of Dermatology, Jinan Central Hospital affiliated to Shandong University, Jinan, Shandong Province, ۲۵۰۰۱۳, China
College of Life Science, Shandong Normal University, Jinan Shandong Province, ۲۵۰۰۱۴, China
Central Laboratory, Jinan Central Hospital affiliated to Shandong University, Jinan, Shandong Province, ۲۵۰۰۱۳, China
چکیده
Objective(s):Several investigations have revealed that caspase-۱۴ is responsible for the epidermal differentiation and cornification, as well as the regulation of moisturizing effect. However, the precise regulation mechanism is still not clear. This study was aimed to investigate the expression of caspase-۱۴ in filaggrin-deficient normal human epidermal keratinocytes (NHEKs) and to explore the possible mechanism that contributes to the regulation of caspase-۱۴. Materials and Methods:The filaggrin-deficient NHEKs were induced by transfection with lentivirus (LV) vector encoding small hairpin RNAs (shRNA). The inhibitors SB۲۰۳۵۸۰, PD۹۸۰۵۹ and SP۶۰۰۱۲۵ were used for suppressing the expression of p۳۸ mitogen-activated protein kinase (MAPK), p۴۴/۴۲ MAPK and stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK). The expression of filaggrin, p۳۸ MAPK, p۴۴/۴۲ MAPK and SAPK/JNK, caspase-۱۴, keratin۱and keratin۲ were detected by western blot. Results:In filaggrin-deficient NHEKs, the expression of p۳۸, p۴۴/۴۲ MAPK and SAPK/JNK and caspase-۱۴ were significantly decreased. The inhibition of p۳۸ and SAPK/JNK reduced the expression of caspase-۱۴, while the p۴۴/۴۲ MAPK showed no consistent effects. Moreover, the filaggrin knockdown decreased the expression of keratin۲, but had no effects on the level of keratin۱. Conclusion: The decreased expression of caspase-۱۴ in filaggrin-deficient NHEKs may be induced by the inactivation of MAPK signaling pathway. These provide a novel perspective to understand the mechanism for the protective effects of filaggrin and caspase-۱۴ on skin barrier function.کلیدواژه ها
Caspase-۱۴, Filaggrin, MAPK signaling pathway Skin barrierاطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.