Effect of ischemic preconditioning on the expression of c-myb in the CA۱ region of the gerbil hippocampus after ischemia/reperfusion injury

Objective(s): In the present study, we investigated the effect of ischemic preconditioning (IPC) on c-myb immunoreactivity as well as neuronal damage/death after a subsequent lethal transient ischemia in gerbils. Materials and Methods: IPC was subjected to a ۲ min sublethal ischemia and a lethal transient ischemia was given ۵ min transient ischemia. The animals in all of the groups were given recovery times of ۱ day, ۲ days and ۵ days and we examined change in c-myb immunoreactivity as well as neuronal damage/death in the hippocampus induced by a lethal transient ischemia. Results:A lethal transient ischemia induced a significant loss of cells in the stratum pyramidale (SP) of the hippocampal CA۱ region at ۵ days post-ischemia, and this insult showed that c-myb immunoreactivity in cells of the SP of the CA۱ region was significantly decreased at ۲ days post-ischemia and disappeared at ۵ days post-ischemia. However, IPC effectively prevented the neuronal loss in the SP and showed that c-myb immunoreactivity was constitutively maintained in the SP after a lethal transient ischemia. Conclusion: Our results show that a lethal transient ischemia significantly decreased c-myb immunoreactivity in the SP of the CA۱ region and that IPC well preserved c-myb immunoreactivity in the SP of the CA۱ region. We suggest that the maintenance of c-myb might be related with IPC-mediated neuroprotection after a lethal ischemic insult.

کلیدواژه ها:

c-myb ، Cells in stratum pyramidale ، Delayed neuronal death ، Ischemic preconditioning ، Ischemia-reperfusion ، protection