miR-۲۶b enhances radiosensitivity of hepatocellular carcinoma cells by targeting EphA۲
- سال انتشار: 1395
- محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 19، شماره: 8
- کد COI اختصاصی: JR_IJBMS-19-8_015
- زبان مقاله: انگلیسی
- تعداد مشاهده: 181
نویسندگان
Department of Oncology, Third Xiangya Hospital, Central South University, NO.۱۳۸, Tong Zi Po Road, Yue Lu District, Chang Sha, Hunan Province, China ۴۱۰۰۱۳
Department of Oncology, Third Xiangya Hospital, Central South University, NO.۱۳۸, Tong Zi Po Road, Yue Lu District, Chang Sha, Hunan Province, China ۴۱۰۰۱۳
Department of Oncology, Third Xiangya Hospital, Central South University, NO.۱۳۸, Tong Zi Po Road, Yue Lu District, Chang Sha, Hunan Province, China ۴۱۰۰۱۳
چکیده
Objective(s): Although low-dose radiotherapy (RT) that involves low collateral damage is more suitable for hepatocellular carcinoma (HCC) than traditional high-dose RT, but to achieve satisfactory therapeutic effect with low-dose RT, it is necessary to sensitize HCC cells to irradiation. This study was aimed to determine whether radiosensitivity of HCC cells can be enhanced using miR-۲۶b by targeting erythropoietin producing human hepatocelluar A۲ (EphA۲). Materials and Methods: The levels of miR-۲۶b and EphA۲ expression in multiple HCC cell lines were assessed by qPCR and western blotting, respectively, and compared with those in a hepatic cell line. HCC ۹۷H cells were transfected with miR-۲۶b mimics, EphA۲-ShRNA or EphA۲ over-expression vector before exposure to low-dose irradiation. Results:Different degrees of miR-۲۶b down-regulation and EphA۲ up-regulation were observed in all HCC cell lines, among which the HCC ۹۷H cell line expressed the lowest level of miR-۲۶b and highest level of EphA۲. EphA۲ was verified as the target of miR-۲۶b by dual luciferase reporter assay. HCC ۹۷H cells transfected with miR-۲۶b mimics or EphA۲-ShRNA reduced the expression of EphA۲ protein, with significantly lower cell proliferation rate and cell invasion ability and higher apoptosis rate in response to low-dose irradiation than those in the non-transfected cells. These results were reversed after EphA۲ was overexpressed by transfection with the EphA۲ overexpression vector. Co-transfection with miR-۲۶b mimics and EphA۲ overexpression vector barely altered EphA۲ expression level and cell response to low-dose irradiation. Conclusion: These data suggest that miR-۲۶b enhances radiosensitivity of HCC ۹۷H cells by targeting EphA۲ protein.کلیدواژه ها
EphA۲, HCC ۹۷H cells, Hepatocellular carcinoma, miR-۲۶, Radiosensitivityاطلاعات بیشتر در مورد COI
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