Antifungal activity of Zataria multiflora essential oil-loaded solid lipid nanoparticles in-vitro condition
- سال انتشار: 1395
- محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 19، شماره: 11
- کد COI اختصاصی: JR_IJBMS-19-11_012
- زبان مقاله: انگلیسی
- تعداد مشاهده: 319
نویسندگان
Department of Horticulture Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Iran
Nanotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
Department of Horticulture Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Iran
Biotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
Department of Horticulture Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Iran
چکیده
Objective(s): The aim of the present study was to prepare, characterize, and evaluate solid lipid nanoparticles (SLNs) containing Zataria multiflora essential oil (ZEO). Materials and Methods: In this study, Z. multiflora essential oil-loaded solid lipid nanoparticles (ZE-SLNs) were prepared to improve its efficiency in controlling some fungal pathogens. SLNs containing Z. multiflora essential oil were prepared by high shear homogenization and ultra sound technique. ZEO-SLNs contained ۰.۰۳% ZEO in ۵% of lipid phase (Glyceryl monostearate-GMS and Precirol® ATO ۵).Tween ۸۰ and Poloxamer ۱۸۸ (۲.۵% w/v) were used as surfactant in the aqueous phase. The antifungal efficacy of ZE-SLNs and ZEO was compared under in vitro conditions. Results:The particle size of ZE-SLNs was around ۲۵۵.۵±۳ nm with PDI of ۰.۳۶۹±۰.۰۵ and zeta potential was about -۳۷.۸±۰.۸ mV. Encapsulation efficacy of ZE-SLNs in crystalline form was ۸۴±۰.۹۲%. The results showed that the ZEO and ZE-SLNs had ۵۴ and ۷۹% inhibition on the growth of fungal pathogens, respectively. The minimum inhibitory concentration (MIC) under in vitro conditions for the ZEO on the fungal pathogens of Aspergillus ochraceus, Aspergillus niger, Aspergillus flavus, Alternaria solani, Rhizoctonia solani, and Rhizopus stolonifer was ۳۰۰, ۲۰۰, ۳۰۰, ۲۰۰, ۲۰۰ and ۲۰۰ ppm, respectively, for ZE-SLNs, it was ۲۰۰, ۲۰۰, ۲۰۰, ۱۰۰, ۵۰ and ۵۰ ppm. The antifungal efficacy of ZE-SLNs was significantly more than ZEO. Conclusion: Our results showed that the SLNs were suitable carriers for Z. multiflora essential oil in controlling the fungal pathogens and merits further investigation.کلیدواژه ها
Essential oil, Minimum inhibitory, Solid lipid nanoparticles, Zataria multifloraاطلاعات بیشتر در مورد COI
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