The cytotoxic effect of memantine and its effect on cytoskeletal proteins expression in metastatic breast cancer cell line

  • سال انتشار: 1396
  • محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 20، شماره: 1
  • کد COI اختصاصی: JR_IJBMS-20-1_007
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 215
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نویسندگان

Sima Seifabadi

Applied Physiology Research Center, Cardiovascular Research Institute, Department of Physiology, Isfahan University of Medical Sciences, Isfahan, Iran

Golnaz Vaseghi

Isfahan Cardiovascular Research Center, Cardiovascular Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran

Shaghayegh Haghjooy Javanmard

Applied Physiology Research Center, Cardiovascular Research Institute, Department of Physiology, Isfahan University of Medical Sciences, Isfahan, Iran

Elham Omidi

Applied Physiology Research Center, Cardiovascular Research Institute, Department of Physiology, Isfahan University of Medical Sciences, Isfahan, Iran

Mohammadhasan Tajadini

Applied Physiology Research Center, Cardiovascular Research Institute, Department of Physiology, Isfahan University of Medical Sciences, Isfahan, Iran

Bahareh Zarrin

Applied Physiology Research Center, Cardiovascular Research Institute, Department of Physiology, Isfahan University of Medical Sciences, Isfahan, Iran

چکیده

Objective(s):Breast cancer is an important leading cause of death from cancer. Stathmin and tau proteins are regulators of cell motility, and their overexpression is associated with the progression and bad prognosis of breast cancer. Memantine, an N-methyl-D-aspartate (NMDA) receptor antagonist, is the potential inhibitor of tau protein in neurons. This study determines the effect of memantine on breast cancer cell migration and proliferation, tau and stathmin gene expression in cancer cells and its synergistic effect with paclitaxel.   Materials and Methods: The cell proliferation was evaluated by MTT assay and for this purpose, MCF-۷ breast cancer cells were treated with various concentration of memantine (۲, ۲۰ and ۱۰۰ μg/ml). Tau and stathmin mRNA expression was evaluated through quantitative real time RT-PCR method. The migration of cancer cells treated with memantine for ۲۴ hr was compared to non-treated cells using an in vitro transmembrane migration assay. Results: Incubation of breast cancer cells with memantine resulted in a dose dependent reduction in cell survival (P=۰.۰۰۰۱). Paclitaxel (۱۰۰ nM) showed synergistic effect with memantine (P=۰.۰۰۰۱). Memantine significantly decreased tau and stathmin mRNA expression (by RT-PCR), so that ۱۰۰ µmol/l of memantine decreased tau and stathmin expression by ۴۶% (P=۰.۰۳۴۱) and ۳۳% (P=۰.۰۴۳), respectively. Migration of cells was also decreased by memantine (P=۰.۰۰۰۱). Conclusion: The presented data shows that memantine reduced mRNA levels of tau and stathmin proteins and also reduced cellular migration.

کلیدواژه ها

Breast Cancer, Memantine, Metastasis, Paclitaxel, Stathmin, Tau protein

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