A novel medium-throughput biological assay system for HTLV-۱ infectivity and drug discovery
- سال انتشار: 1396
- محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 20، شماره: 10
- کد COI اختصاصی: JR_IJBMS-20-10_006
- زبان مقاله: انگلیسی
- تعداد مشاهده: 333
نویسندگان
Department of Microbiology, Faculty of Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran
Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Department of Virology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Virology Department, Pasteur Institute of Iran, ۶۹ Pasteur Ave ۱۳۱۶۹۴۳۵۵۱. Tehran, Iran
چکیده
Objective(s): Here, a reporter cell line containing two reporter vectors were developed, in order to monitor the Human T-Lymphotropic Virus type۱(HTLV-۱) infectivity and the cell viability simultaneously. Materials and Methods: The reporter cell line was constructed by stably transfected baby hamster's kidney cell line (BHK-۲۱), with the genomes expressing two different reporters in separate plasmids.The first reporter gene is transactivated by the HTLV-۱ tax protein, while the second reporter is continuously expressed when introduced into a mammalian cell. In order to show its functionality, the effect of the drug mix on HTLV-۱ was assayed by this system and was compared to the results obtained by other methods. Results: HTLV-۱ reporter cell line was found to produce high level of luciferase when co-cultured with MT-۲ and Hut-۱۰۲ cells but not with Jurkat cell. Moreover, the combination therapy against HTLV-۱ can reduce luciferase expression of the cell when co-cultured with MT-۲ and Hut-۱۰۲ comparable to the ELISA (R=۰.۹۳۲, P-value =۰.۰۰۲). In addition, the results revealed the superiority of the present system over the molecular methods. Conclusion: The results demonstrated that the biological assay system is a beneficial tool for the medium-throughput anti-HTLV-۱ drug screening and inhibitory effect.کلیدواژه ها
Biological Assay, Drug screening, HTLV-۱, Hut۱۰۲, Luciferase, Reporter geneاطلاعات بیشتر در مورد COI
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