The effects of anti-Fas ribozyme on T lymphocyte apoptosis in mice model with chronic obstructive pulmonary disease
- سال انتشار: 1396
- محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 20، شماره: 10
- کد COI اختصاصی: JR_IJBMS-20-10_005
- زبان مقاله: انگلیسی
- تعداد مشاهده: 123
نویسندگان
Department of Respiratory Medicine, the Affiliated Shenzhen Longgang Center Hospital, Zunyi Medical University, Shenzhen City, Guangdong Province, China
Zhuhai Campus of Zunyi Medical University, Zhuhai City, Guangdong Province, China
Department of Respiratory Medicine, the Affiliated Shenzhen Longgang Center Hospital, Zunyi Medical University, Shenzhen City, Guangdong Province, China
Department of Respiratory Medicine, the Affiliated Shenzhen Longgang Center Hospital, Zunyi Medical University, Shenzhen City, Guangdong Province, China
Department of Respiratory Medicine, the Affiliated Shenzhen Longgang Center Hospital, Zunyi Medical University, Shenzhen City, Guangdong Province, China
چکیده
Objective(s): In this study, we aimed to investigate the effects of anti-Fas ribozyme on the apoptosis of T lymphocytes (T cells) in mice model with chronic obstructive pulmonary disease (COPD). Materials and Methods: Male ۶-week-old C۵۷BL/۶ mice were used to establish the COPD model by exposure to cigarette smoke. The COPD mice were sacrificed for spleen dissection and T cell isolation. T cells were randomly divided into four groups (n=۱۰ per group). Group A was used as the control. B, C, and D groups were transfected with empty lentivirus, anti-Fas ribozyme, and an anti-Fas ribozyme mutant, respectively. The expression of Fas mRNA and protein in the T cells were evaluated using qPCR and Western blot, respectively. Flow cytometry was used to evaluate the apoptosis of CD۴＋T cells and calculate the ratio of CD۴+ to CD۸+ T cells (CD۴＋/CD۸＋). Results: Anti-Fas ribozyme significantly inhibited the expression of Fas in the T cells of COPD mice. In addition, the number of apoptotic CD۴＋ T cells and CD۴＋/CD۸＋of the C and D groups were significantly lower and higher than those of group A, respectively (P< ۰.۰۵). The apoptotic CD۴＋T cells and CD۴＋CD۸＋of the C group were significantly lower and higher than those of group D, respectively (P< ۰.۰۵). Conclusion: Anti-Fas ribozyme significantly inhibited the expression of Fas, increased CD۴+/ CD۸+, and inhibited the apoptosis of T cells in COPD mice.کلیدواژه ها
Apoptosis, Anti-Fas ribozyme, COPD mice model, Fas, T cellsاطلاعات بیشتر در مورد COI
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