Effect of fetal and adult bovine serum on pyocyanin production in Pseudomonas aeruginosa isolated from clinical and soil samples

سال انتشار: 1396
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 184

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شناسه ملی سند علمی:

JR_IJBMS-20-12_007

تاریخ نمایه سازی: 28 مهر 1400

چکیده مقاله:

Objective(s): Pyocyanin is a blue-greenish redox-active pigment, produced by Pseudomonas aeruginosa, with a wide range of biological and biotechnological applications. Pyocyanin biosynthesis is regulated by the quorum-sensing (QS) system in which the expression of QS genes and QS-controlled virulence genes may be affected by serum as a complex medium. In the current study, effects of adult bovine serum (ABS) and fetal bovine serum (FBS) on the production of pyocyanin were examined in order to develop it. Materials and Methods: The presence of pyocyanin-producing specific genes and proteins in clinical and soil isolates of P. aeruginosa was confirmed using PCR and SDS-PAGE. Isolates were inoculated to media containing different concentrations of complement-active/-inactivated ABS or FBS and pyocyanin concentration was measured by spectrophotometry. Extracted pigment was characterized by using UV-Visible spectrophotometry. Titration of ABS antibodies against studied isolates was performed by the tube agglutination test. Results: Adding ABS to P. aeruginosa culture medium decreased pyocyanin production compared to the control, while its production increased in FBS-containing media (۱۱۳.۲۱±۲.۵۸۱ vs. ۵۵.۲۶±۰.۸۲۷ μg.ml-۱ and ۱۲۶.۸۰±۲.۰۳۶ vs. ۳۰.۵۶±۰.۳۸۲ μg.ml-۱ of C۱۱ and E۸ pyocyanin concentration in the presence of ۱۰% FBS vs. control, respectively). Conclusion: In this study, due to the presence of inhibitors such as complement proteins and antibodies in ABS samples, the use of FBS devoid of antibodies was effective to increase pyocyanin production in studied isolates.

نویسندگان

Aylin Moayedi

Department of Microbiology, Tehran North Branch, Islamic Azad University, Tehran, Iran

Jamileh Nowroozi

Department of Microbiology, Tehran North Branch, Islamic Azad University, Tehran, Iran

Abbas Akhavan Sepahy

Department of Microbiology, Tehran North Branch, Islamic Azad University, Tehran, Iran

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  • Frank DW. The exoenzyme S regulon of Pseudomonas aeruginosa. Mol ...
  • Budzikiewicz H. Secondary metabolites from fluorescent pseudomonads. FEMS Microbiol Rev ...
  • Lau GW, Hassett DJ, Ran H, Kong F. The role ...
  • Mavrodi DV, Bonsall RF, Delaney SM, Soule MJ, Phillips G, ...
  • Whiteley M, Lee KM, Greenberg E. Identification of genes controlled ...
  • Gross H, Loper JE. Genomics of secondary metabolite production by ...
  • Muller M. Pyocyanin induces oxidative stress in human endothelial cells ...
  • Laursen JB, Nielsen J. Phenazine natural products: biosynthesis, synthetic analogues, ...
  • Rahme LG, Ausubel FM, Cao H, Drenkard E, Goumnerov BC, ...
  • Anjaiah V, Koedam N, Nowak-Thompson B, Loper JE, Höfte M, ...
  • Kerr J, Taylor G, Rutman A, Høiby N, Cole P, ...
  • Rabaey K, Verstraete W. Microbial fuel cells: novel biotechnology for ...
  • Ohfuji K, Sato N, Hamada-Sato N, Kobayashi T, Imada C, ...
  • Hassani HH, Hasan HM, Al-Saadi A, Ali AM, Muhammad MH. ...
  • Zhao J, Wu Y, Alfred A, Wei P, Yang S. ...
  • Kruczek C, Qaisar U, Colmer‐Hamood JA, Hamood AN. Serum influences ...
  • Essar D, Eberly L, Hadero A, Crawford I. Identification and ...
  • Silby MW, Winstanley C, Godfrey SA, Levy SB, Jackson RW. ...
  • Chaerun SK, Tazaki K, Asada R, Kogure K. Bioremediation of ...
  • Vives-Flórez M, Garnica D. Comparison of virulence between clinical and ...
  • El-Amine Bendaha M, Mebrek S, Naimi M, Tifrit A, Belaouni ...
  • Al-Hinai A, Al-Sadi A, Al-Bahry S, Mothershaw A, Al-Said F, ...
  • Karatuna O, Yagci A. Analysis of quorum sensing‐dependent virulence factor ...
  • Mohammed HA, Yossef HS, Mohammad FI. The cytotoxicity effect of ...
  • Vinckx T, Wei Q, Matthijs S, Cornelis P. The Pseudomonas ...
  • Subramaniam L. Rapid diagnosis of Pseudomonas aeruginosa infection by demonstration ...
  • El-Fouly M, Sharaf A, Shahin A, El-Bialy HA, Omara A. ...
  • Juhas M, Wiehlmann L, Huber B, Jordan D, Lauber J, ...
  • Wu L, Estrada O, Zaborina O, Bains M, Shen L, ...
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