Samareh Kamali Sadeghian - Department of Clinical Sciences, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran.
Mahmood Ahmadi-hamedani - Department of Clinical Sciences, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran
Mohammad Hassan Yousefi - Department of Basic Sciences, Faculty of Veterinary Science, Semnan University, Semnan
Reza Narenji Sani - Department of Clinical Sciences, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran

BACKGROUND: It is essential to minimize the effect of time and temperature on the serum biochemical param-eters and determine the stability limits of each analyte in pre-centrifuged blood samples.OBJECTIVES: This study aimed to investigate the stability limits of ۱۰ analytes in Turkoman racehorses blood samples stored in different temperatures and time points.METHODS: The whole blood samples from healthy horses (n=۱۰) were stored for ۲ h (baseline), ۶, ۱۲, ۲۴, and ۴۸ h at ۲۵ºC or ۴ºC. The commercial kits (Parsazmoon, Tehran, Iran) were used for the samples analysis.RESULTS: Albumin (ALB), total bilirubin (TB), and phosphorous (P) exhibited remarkable changes at ۲۵ºC. The storage time for as long as ۱۲ h at ۲۵ºC had no significant effect on urea, total protein (TP), lactate dehydrogenase (LDH), creatinine, and alkaline phosphatase (ALP). The stability of alanine transaminase (ALT) in serum samples stored at ۲۵ºC was for ۲۴ h and for LDH was for ۴۸ h at ۴ºC. Aspartate transaminase (AST) was the most unstable analyte at different storage times at both temperatures. Urea, TP, ALB, TB, and P were stable at ۴ºC for as long as ۶ h. Creatinine and ALP were affected by ۲۴ and ۴۸ h storage times at both temperatures. There was a significant difference (P<۰.۰۵) in AST and ALT activities between two temperatures. No significant difference was observed in creatinine, urea, and TB concentrations between two storage temperatures at any of the storage times.CONCLUSIONS: This study showed that some analytes have acceptable stability in the clotted blood samples stored at ۴°C for ۶ h.

Biochemical parameters, Pre-centrifuged blood samples, Stability limits, Storage time and tem-perature, Turkoman racehorse