Histomorphometric evaluation of mice testicular tissue following short- and long-term effects of lipopolysaccharide-induced endotoxemia
- سال انتشار: 1397
- محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 21، شماره: 1
- کد COI اختصاصی: JR_IJBMS-21-1_008
- زبان مقاله: انگلیسی
- تعداد مشاهده: 125
نویسندگان
Graduate Student of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
Graduate Student of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
چکیده
Objective(s):Lipopolysaccharide (LPS)-induced endotoxemia is known to cause male infertility. This study was designed to explore the effects of bacterial LPS on histomorphometric changes of mice testicular tissues. Materials and Methods: In experiment ۱, a pilot dose responsive study was performed with mice that were divided into five groups, receiving ۳۶۰۰۰, ۱۸۰۰۰, ۹۰۰۰, and ۶۷۵۰ µg/kg body weight (B.W) of LPS or only saline (control). White blood cells (WBC) were observed for ۳ days after LPS inoculation. In experiment ۲, two groups of mice were treated with ۶۷۵۰ µg/kg B.W of LPS or only saline (control). Five cases from each experimental group were sacrificed at ۳, ۳۰, and ۶۰ days after LPS inoculation. Left testes were fixed in Bouin’s solution, and stained for morphometrical assays. Results: Time-course changes of WBC obtained from different doses of LPS-treated mice showed that inoculation of ۶۷۵۰ µg/kg B.W produced a reversible endotoxemia that lasts for ۷۲ hr and so it was used in the second experiment. In experiment ۲, during the first ۳ days, no significant changes were observed in the evaluated parameters instead of seminiferous tubules diameter. Spermatogenesis, Johnsen’s score, meiotic index, and epithelial height were significantly affected at ۳۰th day. However, complete recovery was only observed for the spermatogenesis at day ۶۰. Interestingly, deleterious effects of LPS on spermatogonia were only seen at ۶۰th day (P< ۰.۰۵). Conclusion: Endotoxemia induced by LPS has long-term detrimental effects on spermatogonia and later stage germ cells, which are reversible at the next spermatogenic cycle.کلیدواژه ها
Endotoxemia Lipopolysaccharide, Meiotic index, Spermatogenesis Spermatogoniaاطلاعات بیشتر در مورد COI
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