The cucurbitacins D, E, and I from Ecballium elaterium (L.) upregulate the LC۳ gene and induce cell-cycle arrest in human gastric cancer cell line AGS
- سال انتشار: 1397
- محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 21، شماره: 3
- کد COI اختصاصی: JR_IJBMS-21-3_004
- زبان مقاله: انگلیسی
- تعداد مشاهده: 184
نویسندگان
Department of Cell & Molecular Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran
Department of Cell & Molecular Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran
Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
چکیده
Objective(s): Cucurbitacins exhibit a range of anti-cancer functions. We investigated the effects of cucurbitacins D, E, and I purified from Ecballium elaterium (L.) A. Rich fruits on some apoptotic and autophagy genes in human gastric cancer cell line AGS. Materials and Methods: Using quantitative reverse transcription PCR (qRT-PCR), the expression of LC۳, VEGF, BAX, caspase-۳, and c-MYC genes were quantified in AGS cells ۲۴ hr after treatment with cucurbitacins D, E, and I at concentrations ۰.۳, ۰.۱ and ۰.۵ μg/ml, respectively. Cell cycle and death were analyzed by flowcytometry. Results: Purified cucurbitacins induced sub-G۱ cell-cycle arrest and cell death in AGS cells and upregulated LC۳mRNA effectively, but showed a very low effect on BAX, caspase-۳, and c-MYC mRNA levels. Also after treatment with cucurbitacin I at concentration ۰.۵ μg/ml, VEGF mRNA levels were increased about ۴.۴ times. Pairwise comparison of the effect of cucurbitacins D, E, and I on LC۳ mRNA expression showed that the cucurbitacin I effect is ۱.۳ and ۱.۱ times that of cucurbitacins E and D, respectively; cucurbitacin D effect is ۱.۲ times that of cucurbitacin E (P-value < ۰.۰۵). In silico analysis showed that among autophagy genes, LC۳ has an important gastric cancer rank relation. Conclusion: Cucurbitacins D, E, and I purified from E. elaterium fruits upregulate LC۳ and induce sub-G۱ cell-cycle arrest and cell death in human gastric cancer cell line AGS. Cucurbitacin I effect on LC۳ mRNA expression is significantly more than that of cucurbitacins E and D.کلیدواژه ها
Apoptosis, Autophagy, Cell cycle, Cucurbitacins, Stomach Neoplasmsاطلاعات بیشتر در مورد COI
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