Study of class ۱ integrons in multidrug-resistant uropathogenic Escherichia coli isolated from different hospitals in Karachi
- سال انتشار: 1397
- محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 21، شماره: 10
- کد COI اختصاصی: JR_IJBMS-21-10_014
- زبان مقاله: انگلیسی
- تعداد مشاهده: 121
نویسندگان
Dow University of Health Sciences, Karachi, Pakistan
Barrett Hodgson University, Karachi, Pakistan
Pakistan Council of Scientific & Industrial Research Laboratories Complex, Karachi, Pakistan
Department of Microbiology, University of Karachi, Karachi, Pakistan
Dada Bhoy Institute of Higher Education Commission, Karachi, Pakistan
Dada Bhoy Institute of Higher Education Commission, Karachi, Pakistan
چکیده
Objective(s): Escherichia coli is the key pathogen in the family producing ESBL (extended spectrum β-lactamase) and associated with community-acquired infections. Therefore, this study was planned to determine the antibiotic susceptibility pattern of uropathogenic E. coli, prevalence of the ESBL gene group and class ۱ integrons.Materials and Methods: Clinical isolates of uropathogenic E. coli were isolated from different hospitals of Karachi. Antibiotic susceptibility test was performed by Kirby-Bauer Methods. Presence of β– lactamases genes (CTX, TEM, and SHV) and integron ۱ were identified by polymerase chain reaction (PCR).Results: Out of ۵۰۰, ۱۰۵ isolates were identified as multi-drug resistant (MDR) uropathogenic E. coli. The subject MDR isolates showed the highest resistance to aztreonam, amoxil/ clavulanic acid, ampicillin, cotrimoxazole, ceftriaxone, cefipime, and cefuroxime. Genetic analysis showed that the majority of the MDR E. coli carry CTX M۱ (۵۷.۱%) followed by TEM (۳۳.۳%) and SHV (۹.۵%). Moreover, ۷۹% of MDR E. coli harbored class ۱ integrons, whereas all three conserved genes for class ۱ integrons were present in ۵۸% of MDR E. coli. Conclusion: This study is helpful to provide information regarding the antibiotic susceptibility pattern, distribution ESBLs and class ۱ integrons among uropathogenic E. coli.کلیدواژه ها
Class ۱ integrons, ESBLs, MDR E. coli, Multidrug resistance, Uropathogenic E. coliاطلاعات بیشتر در مورد COI
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