Neuroprotective effects of Pinus eldarica in a mouse model of pentylenetetrazole-induced seizures

Objective: Oxidative stress has pernicious effects on the brain. Pinus eldarica has antioxidant properties. We explored neuroprotective effect of P. eldarica against pentylenetetrazole (PTZ)-induced seizures.Materials and Methods: Male mice (BALB/c) were grouped as control, PTZ, Soxhlet (Sox) ۱۰۰, Sox ۲۰۰, Macerated (Mac) ۱۰۰ and Mac ۲۰۰ groups. Sox and Mac extracts (۱۰۰ and ۲۰۰ mg/kg) were injected during ۷ days. Delay in onset of minimal clonic seizure (MCS) and generalized tonic- clonic seizure (GTCS) was measured. Number of dark neurons (DN) and levels of oxidative stress indicators in the hippocampus were evaluated.Results: Onset of MCS and GTCS was later in groups treated with the extracts than the PTZ group (p<۰.۰۱ and p<۰.۰۰۱). Number of DN in the hippocampus in the PTZ group was higher than the control group (p<۰.۰۰۱) while in the extract groups, was lower than the PTZ group (p<۰.۰۵, p<۰.۰۱ and p<۰.۰۰۱). MDA level was higher whereas total thiol level and activity of SOD and CAT were lower (p<۰.۰۰۱) in the PTZ group than the control group. MDA level in the Sox ۱۰۰ (p<۰.۰۱), Sox ۲۰۰ (p<۰.۰۰۱) and Mac ۲۰۰ (p<۰.۰۱) groups was less than the PTZ group. Total thiol level in the Sox ۲۰۰ (p<۰.۰۰۱), SOD in the Sox ۱۰۰ (p<۰.۰۵), Sox ۲۰۰, and Mac ۲۰۰ and CAT in the Sox ۲۰۰ (p<۰.۰۰۱) groups were higher than the PTZ group.  Conclusion: P. eldarica prevented neuronal death and reduced seizures caused by PTZ via improving brain oxidative stress.

کلیدواژه ها:

Pinus eldarica ، Pentylenetetrazole ، Oxidative stress ، Dark neurons