Effects of synthetic silymarin-PLGA nanoparticles on M۲ polarization and inflammatory cytokines in LPS-treated murine peritoneal macrophages

  • سال انتشار: 1400
  • محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 24، شماره: 10
  • کد COI اختصاصی: JR_IJBMS-24-10_016
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 194
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نویسندگان

Mojgan Azadpour

Research Center of Pediatric Infectious Diseases, Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran

Mohammad Morad Farajollahi

Department of Medical Biotechnology, School of Allied Medical Sciences, Iran University of Medical Sciences,Tehran, Iran

Hassan Dariushnejad

Department of Medical Biotechnology, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran

Ali Mohammad Varzi

Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran

Amir Varezardi

Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran

Mitra Barati

Research Center of Pediatric Infectious Diseases, Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran

چکیده

Objective(s): Silymarin (SM) is a natural antioxidant compound with good anti-inflammatory effects, but its poor water solubility restricts its usage. Today, nanomaterial compounds (such as PLGA Poly D, L-lactic-co-glycolic acid) can provide a proper drug delivery system and help improve the accessibility of bioactive compounds to cells and tissues. Materials and Methods: In this study, PLGA nanoparticles (NPs) containing SM (SM-PLGA) were synthesized and characterized and their biological effects were evaluated on M۲ macrophage polarization to regulate inflammation. SM-PLGA NPs were fabricated by the oil in water emulsion (O/W) method. Macrophages (MQs) were isolated from mouse peritoneum by the cold RPMI lavage protocol. Primary mouse MQ cells were treated by SM and SM-PLGA NPs and then stimulated with lipopolysaccharide (LPS). M۲ polarization was evaluated by measurements of cytokine secretion levels (TNF-α, IL۱-β, and IL-۱۰), flow cytometry markers (F۴/۸۰, CD۱۱b, CD۳۸, and CD۲۰۶), and the expression of specific proteins (M۲ Ym۱ and Fizz۱).Results: SM-PLGA characterization showed that NPs were fabricated in the desired form. SM and SM-PLGA decreased pro-inflammatory cytokines (TNF-α and IL۱-β) and increased IL۱۰ as an anti-inflammatory cytokine. On the other hand, the M۲-associated markers and proteins increased following treatment with SM and SM-PLGA. Post-hoc analysis indicated that these changes were more pronounced in the SM-PLGA group.Conclusion: This study revealed that SM-PLGA could markedly promote M۲ polarization, thereby providing a valuable medical approach against sepsis and septic shock.

کلیدواژه ها

Cytokines, Nanoparticles, PLGA compound, Peritoneal macrophages, Silymarin

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