Genome analysis of an enterococcal prophage Entfac.MY

سال انتشار: 1400
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 154

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شناسه ملی سند علمی:

MEDISM22_352

تاریخ نمایه سازی: 8 مهر 1400

چکیده مقاله:

Background and Aim : Bacteriophages (phages) are bacterial intracellular parasites. They are the most abundant organisms in the world and are present in any environment that contains bacteria, including sewage. Lysogenic phages, unlike lytic phages, do not begin to multiply immediately after entering the host and may integrate their genome into a bacterial gene called a prophage. Prophages can have a wide range of phenotypic effects on host bacteria. They can also regulate bacterial populations by altering bacterial gene expression. Enterococcus are gram-positive bacteria whose species cause infections in humans. Recently, Enterococcus has become resistant to a variety of antimicrobials, so that today we see high resistance of Enterococcus to a variety of antibiotics, including vancomycin.Methods : In this study, Enterococcus faecium EntfacMY was isolated from biological samples and its genome was examined.Results : After analyzing the genome of these bacteria, ۲۵۴ prophage genes were identified. The prophage included ۳۹ housekeeping genes, ۴۱ genes related to replication and regulation, ۸۰ genes in the group of structural and packaging genes and ۴۸ genes in the group of lysis. ۴۶ genes with unknown function were also identified.Conclusion : As a result, identification and analysis of prophage genomes can be effective in better understanding their role in the development of bacterial resistance to antibiotics. Identifying and studying prophages can also help develop genetic engineering tools and therapeutic use of bacteriophages. The identified genes were annotated in the DNA Data Bank of Japan.

نویسندگان

Maryam Yazdanizad

Department of Medical Biotechnology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

Ali Akbar Saboor Yaraghi

Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

Ramin Mazaheri Nezhad Fard

Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran