Comparison of Helicobacter pylori by polymerase chain reactions and surface cultivation methods for presenting consumed water tap of selected hospitals in Tehran in ۲۰۲۰

Background and Aim : Helicobacter pylori is the most common gastrointestinal pathogen that more than half of the world's population is infected with this bacterium. Helicobacter pylori is a bacterium that causes gastrointestinal ulcer (chronic gastritis), stomach cancer, lymphoma, and adenocarcinoma. This pathogenic bacterium is associated with an increased risk of gastrointestinal cancer among sludge workers. Epidemiological studies propound person-to-person transmission and environmental transmission through water and food. The aim of this study was to compare polymerase chain reaction (PCR) and surface culture methods for the presence of Helicobacter pylori in consumed water of selected hospitals in Tehran in ۲۰۲۰. Methods : In this study, sample analysis was randomly selected. Samples of consumed water taps of the drinking water distribution system ۲۲ samples and well water ۶ samples were prepared from selected hospitals in different areas of Tehran from September ۵ to November ۲۰, ۲۰۲۰. The samples were collected in sterile bottle according to procedure detailed in national standard methods. In this study, Helicobacter pylori was evaluated using polymerase chain reaction (PCR) and surface culture.Results : This study showed the prevalence of Helicobacter pylori by PCR in two cases. The mean of Helicobacter pylori in consumed water tap and well water samples was ۰.۱۸ ± ۰.۸۵ and ۰.۶۷±۱.۶۳, respectively. The mean of Heterotroph Plate Count in consumed water tap and well water samples was ۰.۰۰ ±۰.۰۰ and ۷.۸۳±۲.۱۰, respectively. The mean of Escherichia coli, Pseudomonas aeruginosa, Clostridium perfringens, Streptococcus faecalis in consumed water tap samples was ۰.۰۰±۰.۰۰ and well water samples was ۰.۰۰ ±۰.۰۰ (as a negative control), ۵.۲۰±۰.۲۲ (as a positive control), and ۲.۲±۰.۲۲, ۲.۲± and ۷.۸۳±۲.۱۰, respectively.Conclusion : So according to our results, Infection control and preventive strategies planning in order to reducing the exposure risk to Helicobacter pylori due to producing a safe water supply is purposed to public health authorities. Evaluation of biological quality (Heterotrophic bacteria, Coliforms, Escherichia coli, Pseudomonas aeruginosa, Clostridium perfringens, Streptococcus faecalis), physicochemical quality of water and Helicobacter pylori are among the strengths and innovations of this research.

کلیدواژه ها:

Drinking water distribution system ، Helicobacter pylori ، Heterotroph Plate Count ، Surface culture ، Polymerase Chain Reaction (PCR) method ، Water well.