Evaluation of antimicrobial activity of dihydropyrimidines and imidazo [۱, ۲-a] pyridine derivatives against pathogenic bacteria from urinary tract infection

Background and Aim : In addition to the problems of antibiotic resistance, resistance to antiseptic compounds has encouraged chemists to find new chemical compounds as antimicrobials. since Urinary tract infection (UTI) is one of the most common bacterial infections. In this study, the susceptibility of isolates of some bacteria was investigated by performing various microbiological methods to the chemicals dihydro-pyrimidines and Imidazo [۱, ۲-a] pyridines derivatives.Methods : In this cross-sectional study, ۳۰ isolates were collected from patients with Urinary tract infections referring to the medical diagnostic laboratory in Mashhad, Iran. Clinical samples were determined by biochemical and microbiology tests. Minimum inhibitory concentration test was used to optimize the best concentration of chemical derivatives, the dihydro-pyrimidine and Imidazo [۱, ۲-a] pyridines, for four strains standard such as Enterococcus faecalis, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa by the using microdilution method. The susceptibility of these strains of bacteria was evaluated and the best combination was selected to examining the clinical samples by agar well diffusion method.Results : In this research, the relative frequency of bacteria isolated from the urine samples in bacterial culture were ۴۰% Escherichia coli, ۲۳.۳% Staphylococcus aureus, ۱۶.۶% Klebsiella pneumonia, ۱۶.۶% Enterobacter spp., and ۳.۳% Pseudomonas aeruginosa. According to the results of MIC, the best compound and concentration was derivative of Imidazo [۱, ۲-a] pyridines and ۱۵ ?g /ml respectively for ۴ standard strains of bacteria, but the effective concentration in the agar well diffusion was ۲۰۰ ?g /ml almost for all UTI bacteria. Conclusion : The results of this study show that derivatives of Imidazo [ ۱,۲-a] pyridines can be used as antibacterial products in Urinary tract infections.

کلیدواژه ها:

Dihydro-pyrimidines ، Imidazo [۱ ، ۲-a] pyridines ، Urinary tract infection ، MIC