Prevalence and Population Structure of Helicobacter pylori Isolates from Patients with Dyspepsia in Isfahan, Iran
- سال انتشار: 1400
- محل انتشار: بیست دومین کنگره میکروب شناسی ایران (مجازی)
- کد COI اختصاصی: MEDISM22_322
- زبان مقاله: انگلیسی
- تعداد مشاهده: 279
نویسندگان
Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
چکیده
Background and Aim : Helicobacter pylori (H. pylori), a spiral-shaped bacterium colonizing the human stomach, is generally acquired in childhood. This pathogen is highly diverse and can be used as genetic markers for predict the history of human migrations. This study was aimed to determine the genetic diversity of H. pylori isolates from patients with dyspepsia by the multilocus sequence typing (MLST), and update data on the prevalence of H. pylori among Iranian dyspeptic patients.Methods : In this descriptive cross-sectional study, ۱۶۵ gastric biopsy specimens were obtained from patients with dyspepsia referred to Dr. Shariati Hospital of Isfahan, Iran from April to July ۲۰۱۸. The status of H. pylori infection was determined by FISH in paraffin-embedded biopsy specimens. MLST of seven housekeeping genes was performed for ۲۰ H. pylori isolates. The phylogenetic tree was plotted using CLC v۸ and iTol software. Results : The overall prevalence of H. pylori infection was ۵۳.۳%. In the results of the analysis of MLST, a total of ۱۴ new STs were recorded. The results of the global analysis showed that all the isolates, with a wide diversity, have a genetic affinity with members of the European population, such as Italy and Russia, and are in the hpEurope haplotype. Conclusion : Given the high prevalence of Helicobacter pylori infection in this region, early and accurate identification of patients seems necessary. Sequence analysis and determination of the origin of the phylogeny of strains can be effective in clinical management and monitoring of risk factors for chronic and recurrence of infection.کلیدواژه ها
Helicobacter pylori, MLST, prevalenceمقالات مرتبط جدید
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