Biochemical and molecular identification of probiotic bacteria isolated from traditional Maragheh buttermilk
- سال انتشار: 1400
- محل انتشار: بیست دومین کنگره میکروب شناسی ایران (مجازی)
- کد COI اختصاصی: MEDISM22_258
- زبان مقاله: انگلیسی
- تعداد مشاهده: 355
نویسندگان
Department of Biology, Faculty of Basic Sciences, University of Maragheh, Maragheh, Iran
Department of Biology, Faculty of Basic Sciences, University of Maragheh, Maragheh, Iran
Department of Microbiology, Faculty of Science, Islamic Azad University of Arak, Arak, Iran
چکیده
Background and Aim : : Probiotics are defined as living microorganisms, when ingested in sufficient amounts provide health benefits for the host. The two main types of probiotics are Lactobacillus and Bifidobacterium. Probiotics can be used in the form of powders, syrups, tablets and fortified foods. Application of probiotics and prebiotics as biotherapeutics is the new field in developing dietary strategies. Methods : To identify the isolated bacteria, microbial and biochemical tests including gram staining, catalase and oxidase activity, sugar fermentation of carbohydrates, antibacterial tests and acid and bile salts resistance were performed. The asparaginase and glutaminase production was evaluated by raid plate assay. Also, bacterial DNA was extracted and ۱۶S rRNA gene was amplified through PCR using universal primers. Results : The results confirmed the isolated bacteria as a probiotic including; gram-positive, catalase- and oxidase-negative, grow at bile salts and acidic pH and no hemolysis. Based on ۱۶S rRNA gene amplification, it was identified as Lactobacillus. Furthermore, the production of therapeutic enzymes asparaginase and glutaminase was verified.Conclusion : The isolated bacteria from traditional buttermilk confirmed as lactobacillus and further experiments about asparaginase and glutaminase production are running.کلیدواژه ها
Buttermilk, Lactobacillus, Probiotic, ۱۶s rRNAمقالات مرتبط جدید
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