Evaluation of doxycycline and zinc oxide nanocomposite films effect on some gram positive and gram negative bacteria

Background and Aim : many researches has been done recently on the production and Evaluation of antibacterial effects of medical nanoparticles because of the prevalence of infections caused by bacteria resistance to one or more antibiotics, lack of proper efficacy and recovery from infection, increased treatment costs and widespread side effects due to the use of common antibiotics. Metal nano oxides such as zinc oxide nanoparticles have been considered in recent years, due to stability in various conditions and generally used as safe materials for humans and animals.Methods : In the present study, disk diffusion method, determination of MIC (Minimum Inhibitory Concentration) and MBC (Minimum Bactericidal Concentration) were used.Results : The MIC obtained for zinc oxide nanocomposite film, doxycycline nanocomposites and zinc oxide-doxycycline nanocomposite for Staphylococcus aureus (۲۹۲۱۳ ATCC) ۰.۴۸, ۰.۰۳ and ۰.۰۰۳۷۵ mg/ml respectively, for Escherichia coli (۳۵۲۱۸ ATCC) were ۰.۹۶, ۰.۴۸ and ۰.۲۴ mg/ml, respectively. Concentrations obtained to express the MBC of bacteria for Staphylococcus aureus were ۰.۹۶, ۰.۰۶, ۰.۰۰۷۵ mg/ml, respectively, and for Escherichia coli were ۱.۹۲, ۰.۹۶ and ۰.۴۸ mg/ml respectively. Which was statistically significant during our studies in different groups (p <۰.۰۵).Conclusion : The antimicrobial effect of nanocomposite films against bacterial pathogens in all studied methods for Staphylococcus aureus is more significant than Escherichia coli. The increase of these effects against two bacteria for films is also in the form of zinc oxide was determined (Zinc oxide-doxycycline nanocomposite more than doxycycline nanocomposite and more than zinc oxide nanocomposite).

کلیدواژه ها:

Antibacterial effect ، Metal nano oxides ، Nanocomposite films ، MIC ، MBC.