Isolation, biochemical and molecular identification of Salmonella isolates from Mazandaran poultry industry submitted to Razi Vaccine and Serum Research Institute

Background and Aim : The genus Salmonella is a group of Gram-negative bacteria belonging to the Enterobacteriaceae family. The importance of research on Salmonella infection in industrial poultry includes irreparable economic losses due to salmonellosis in the poultry industry. Salmonella infection is one of the common major diseases in humans, livestock and poultry. In addition, it is a major cause of humans’ food poisoning. From financial and public health prospective, salmonellosis in poultry, its dominant serotypes and antibiotic resistance are interesting topics, which need continuous research. Especially, if new and fast techniques can detect Salmonella serotype.Methods : The isolates were identified by Culture and Biochemical test, then serotyped by Multiplex PCR.Results : In this study, suspicious were ۱-۱۰ days old broilers sent from ۲۹ herds in Mazandaran province, which were isolated from a total of ۱۱ herds (۳۷.۳۷%) of Salmonella genus. The most magnificent part of this study was the isolation of two Salmonella enteritidis isolates from broiler brains. The isolates were serotyped by Multiplex PCR. The results showed that, they were Salmonella enterica serovar Enteritidis .The most magnificent part of this study was the isolation of two Salmonella enteritidis isolates from broiler brains. Conclusion : The importance of research on Salmonella infection in industrial poultry includes irreparable economic losses due to salmonellosis in the poultry industry. Salmonella infection is one of the common major diseases in humans, livestock and poultry. In addition, it is a major cause of humans’ food poisoning. From financial and public health prospective, salmonellosis in poultry, its dominant serotypes and antibiotic resistance are interesting topics, which need continuous research. Especially, if new and fast techniques can detect Salmonella serotype. In this study, suspicious were ۱-۱۰ days old broilers sent from ۲۹ herds in Mazandaran province, which were isolated from a total of ۱۱ herds (۳۷.۳۷%) of Salmonella genus.

کلیدواژه ها:

Salmonella ، Poultry ، Isolation ، Identification ، Molecular ، Enteritidis