Identification of Loa۲۲ coding gene in the vaccinal Leptospira serovars
- سال انتشار: 1400
- محل انتشار: بیست دومین کنگره میکروب شناسی ایران (مجازی)
- کد COI اختصاصی: MEDISM22_215
- زبان مقاله: انگلیسی
- تعداد مشاهده: 278
نویسندگان
Department of Microbiology, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
Department of Microbiology, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
Department of Microbiology, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
Department of Research and Development, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
چکیده
Background and Aim : Leptospirosis is one of the most important zoonotic diseases, which is caused by the Leptospira. Some Leptospra membrane lipoproteins perform as bacterial virulence factors. The aim of the present study was to molecular identification and investigation the presence of Loa۲۲ gene encoding a membrane lipoprotein in the vaccinal Leptospira serovars.Methods : In the present study, three vaccinal Leptospira serovars including L. Grippotyphosa, L. Canicola and L. Serjoe hardjo as well as a saprophytic serovar, L. Semanerga, were prepared. After DNA extraction by standard phenol-chloroform method and quantitative and qualitative analysis of the extracted DNA, PCR reaction was performed to amplify the Loa۲۲ gene. PCR products were evaluated by electrophoresis on agarose gel and finally sequenced. The resulting sequences were compared using Megalign software and the serovars genetic similarity was investigated.Results : The results of electrophoresis of PCR products showed ۶۷۱ bp bands indicating the presence of Loa۲۲ in vaccinal samples, while no band was formed in the saprophytic sample. The percentage of this gene similarity was high in pathogenic serovars so that the minimum similarity was ۹۷.۳% and the maximum similarity was ۹۹.۶%, which indicates the conservation of Loa۲۲ gene. Conclusion : Loa۲۲ gene is a specific conserved gene of vaccinal Leptospira serovars and can be used as a diagnostic marker to identify these serovars and differentiate them from the saprophytic ones.کلیدواژه ها
Leptospirosis, Vaccinal Leptospira serovars, Molecular identification, Loa۲۲ geneمقالات مرتبط جدید
اطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.