Isolation and characterization of bacteriophage against Klebsiella pneumoniae from Cystic Fibrosis patient

Background and Aim : Today, infections caused by Klebsiella pneumoniae have become a problem in treatment due to the increased proliferation of plasmids carrying antibiotic resistance genes among bacteria of the Enterobacteriaceae family and that is one of the therapeutic problems of Cystic Fibrisis disease. The discovery of novel agents to control Klebsiella pneomoniae infections is urgent due to the broad multidrug-resistance. A phage with the ability to efficiently and specifically lyse bacteria is considered an alternative to control these pathogens. This study aimed to isolate a lytic bacteriophage with the potential to lyse clinical isolates of Klebsiella pneumoniae.Methods : Antimicrobial Susceptibility of clinical Klebsiella pneomoniae Isolates was determined using disk diffusion assays then Water samples were collected from a hospital waste-water in Tehran.The samples were filtered and mixed with an overnight grown culture of Klebsiella pneumoniae in notrient broth . Phage titration, latent period, and burst size measurements were carried out by the double-layer agar method using the Klebsiella pneumoniae. The isolated phage was characterized by Transmission Electron Microscopy (TEM), Spot test was employed to determine the bacterial host range for the isolated phage using eleven multidrug resistant Klebsiella pneumoniae isolates from patients with Cystic Fibrosis and Effectiveness of a phage against Klebsiella pneomoniae biofilms was determined by Minimum Biofilm Eradication Concentration (MBEC) Assay System.Results : The isolated phage belongs to the Myoviridae family and infected seven of eleven tested multidrug-resistant Klebsiella pneumoniae strains.This phage had large burst sizes, efficient rates of adsorption and were stable under different adverse conditions.Conclusion : As the phage was virulent and specific for Klebsiella pneumoniae, and was stable and active at different condition that exhibit a number of properties indicative of potential utility in phage therapy cocktails

کلیدواژه ها:

Bacteriophage ، Cystic fibrosis ، Klebsiella pneumoniae ، Phage therapy