A novel modified peptide derived from DCD-۱L can inhibit the biofilm formation of ESKAPE bacteria
- سال انتشار: 1400
- محل انتشار: بیست دومین کنگره میکروب شناسی ایران (مجازی)
- کد COI اختصاصی: MEDISM22_188
- زبان مقاله: انگلیسی
- تعداد مشاهده: 330
نویسندگان
Department of microbiology, School of medicine, Ahvaz jundishapur university of medical sciences, Ahvaz, Iran
Department of medical laberatory sciences, School of allied medical sciences, Ahvaz jundishapur university of medical sciences, Ahvaz, Iran
Department of microbiology, School of medicine, Tehran university of medical sciences, Tehran, Iran
چکیده
Background and Aim : The high ability to develop biofilm as a critical factor in chronic infections along with high rate of drug-resistant ESKAPE bacteria (Enterococcus faecalis, Staphylococcus aureus, Klebsiella pneumonia, Acinetobacter baumannii, Pseudomonas aeruginosa, and Escherichia coli) highlights the need to identify novel antibiotics. The aim of this study was to assess in vitro and in vivo anti-biofilm activities of A novel modified peptide derived from dermcidin-۱L (mDCD-۱L) against clinical strains. Methods : In vitro antibacterial, anti-adhesive, and anti-biofilm activities of mDCD-۱L against standard strains of ESKAPE bacteria (Enterococcus faecalis; ATCC ۲۹۲۱۲, Staphylococcus aureus; ۲۵۹۲۳, Klebsiella pneumonia; ۷۰۰۶۰۳, Acinetobacter baumannii; ۱۹۶۰۶, Pseudomonas aeruginosa; ۲۷۸۵۳, and Escherichia coli; ۲۵۹۲۲ ) were investigated. Furthermore, a mouse model of catheter-associated biofilm infection was utilized to assess anti-biofilm activity of mDCD-۱L against standard strains of mentioned bacteria.Results : Minimum inhibitory concentration (MIC) of mDCD-۱L was ۴ µg/ml for A. baumannii, P. aeroginosa, E. coli and K. pneumonia and was ۸ µg/ml for S. aureus and E. faecalis. mDCD-۱L also inhibited biofilm formation by ∼ ۵۳% at sub-inhibitory concentration (۱/۴MIC) and ۱۰۰% at ۱/۲ MIC whereas the high value of MBEC (۲۵۶ µg/ml) indicates ability of mDCD-۱L to decrease mass of existing biofilm. Furthermore, in mouse catheter infection model following treatment with mDCD-۱L at MIC, the biofilm was significantly reduced as compared to the untreated control.Conclusion : In the present study antibacterial and antibiofilm function of a novel synthetic cationic antimicrobial peptide (AMP) derived from human anionic AMP DCD-۱L (mDCD-۱L) was demonstrated, suggesting that mDCD-۱L could affective as a potential therapeutic agent against chronic, recurrent biofilm infections caused by ESKAPE bacteria.کلیدواژه ها
Biofilm, Antimicrobial peptide, ESKAPE bacteria, antibacterial, antibiofilmمقالات مرتبط جدید
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