Anti-Quorum sensing activity of Archangium sp. metabolite extract against Pseudomonas aeruginosa
- سال انتشار: 1400
- محل انتشار: بیست دومین کنگره میکروب شناسی ایران (مجازی)
- کد COI اختصاصی: MEDISM22_164
- زبان مقاله: انگلیسی
- تعداد مشاهده: 271
نویسندگان
MSc student, Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, Iran
Associate Professor of Medical Bacteriology, Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, Iran
Associate Professor of Microbial Biotechnology, Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, Iran
چکیده
Background and Aim : Pseudomonas aeruginosa is a common cause of nosocomial and opportunistic infections. Variety of virulence factors present in P. aeruginosa can empower pathogenicity of the bacteria in the host. Most of these pathogenic properties are controlled by a hierarchical system called Quorum Sensing (QS). Myxobacteria are gram-negative rods, producing bioactive secondary metabolites which have antimicrobial activities. In this study, we investigated the effect of Archangium sp. metabolite extract (AME) on biofilm and virulence factors of P. aeruginosa, which are controlled by QS systems. Methods : The minimum inhibitory concentration (MIC) of AME was determined against P. aeruginosa ATCC ۲۷۸۵۳. AME was tested for its effects on the production of P. aeruginosa virulence factors controlled by QS system (biofilm formation, pyocyanin and rhamnolipid production, swarming and twitching motility).Results : The MIC of AME against P. aeruginosa ATCC ۲۷۸۵۳ was ۲.۵ mg/ml. Sub-MIC concentrations demonstrated statistically significant reduction of virulence factors including pyocyanin and rhamnolipid production. Biofilm formation and twitching motility were also reduced after AME treatment.Conclusion : These results indicate that AME inhibited the production of virulence factors and biofilm formation which are under the control of QS systems in P. aeruginosa. Several secondary metabolites produced by Myxobacteria are promising antimicrobial agents. This study shows that Myxobacteria secondary metabolites can have anti-QS activity as well. So, further studies can be focused on the isolation of bioactive molecules with anti-QS activity, to determine their chemical nature and to deduce their mechanisms of anti-QS activities.کلیدواژه ها
Pseudomonas aeruginosa, QS systems, anti-QS activity, Myxobacteriaمقالات مرتبط جدید
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