Investigation of antibacterial properties of borage extract

Background and Aim : Nowadays, finding of alternative antibacterial agents in essential, because of spreading of resistant bacteria compared to antibiotic drugs and problems of their use. The purpose of this investigation is determination of borage (Echium amoenum) antibiotic effects on two human pathogenic bacteria including Staphylococcus aureus and Salmonella typhimurium and four plant pathogenic bacteria including Brenneria nigrifluens, Pseudomonas syringae, Serratia marcescens and Erwinia amylovora in vitro condition.Methods : In the present study, first prepared the extract of aqueous and alcoholic for samples of borage in three dilution (۵۰ mg/ml, ۱۰۰mg/ml and ۲۰۰ mg/ml) of dimethyl sulfoxide (DMSO) solvent. Evaluation of antibacterial properties borage extracts was performed by disk diffusion. Diameter of the transparent halos caused by inhibition of bacterial growth was evaluated.Results : The extract of borage caused a growth inhibition zone in all studied bacterial species in this survey. The smallest diameter zone was related to concentration of ۵۰ mg/ml in both of aqueous and alcoholic extracts. The results of evaluation of zone indicated that in higher concentrations of borage extract, the diameter of growth zone will be increased. Conclusion : The results of this study showed the borage extract has antibacterial properties and we can be hopeful to make some useful drugs mixed with plant origin and much less side effects to confronting of bacteria.