Shiva Jahanbakhshi - Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences

Background and Aim : Salmonella is a facultative anaerobic gram-negative rod-shaped bacteria that belong to the Enterobacteriaceae family. Extensive use of antimicrobial agents such as Cotrimoxazole in medicine and veterinary has been associated with the rising of antimicrobial resistance. In the current study, we focused on the assessment of the frequency of cotrimoxazole resistance genes among Salmonella spp.Methods : forty-one isolates were identified from Mar.۲۰۱۹ to Apr.۲۰۲۰ in Imam Khomeini hospital of Tehran. Antibiotic susceptibility of isolates was done through the Kirby-Bauer method. The sul۱, sul۲, sul۳, dfrA۱, dfrA۵, and Int۱, genes were detected by Multiplex-PCR. Results : among ۴۱ isolates, ۱۴ isolates (۲۹%) were resistant to the cotrimoxazole. The frequency of dfra۱ and dfra۵ is ۱۰% (۴ isolates) and ۱۰% (۴ isolates) respectively. The frequency of other genes includes sul۱, sul۲ and sul۳ are ۲۵% (۱۰ isolates), ۱۹% (۸ isolates) and ۰% (۰ isolates) respectively.Conclusion : data have shown that the antimicrobial resistance increased and one-third of Salmonella isolates have a Cotrimoxazole resistance gene that illustrated the accumulation of resistance genes among Salmonella spp.

cotrimoxazole, Salmonella, antimicrobial resistance