Expression of RND efflux pumps mediated antibiotic resistance in Pseudomonas aeruginosa Clinical Strains
محل انتشار: بیست دومین کنگره میکروب شناسی ایران (مجازی)
سال انتشار: 1400
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 160
نسخه کامل این مقاله ارائه نشده است و در دسترس نمی باشد
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
MEDISM22_116
تاریخ نمایه سازی: 8 مهر 1400
چکیده مقاله:
Background and Aim : Resistance-Nodulation-Division (RND) efflux pumps are responsible for multidrug resistance in Pseudomonas aeruginosa. The present study aimed to evaluate the overexpression of RND efflux pumps and its role in the antibiotic resistance of P. aeruginosa clinical isolates. A number of ۱۲۲ isolates were obtained from three military hospitals in Tehran, Iran. Methods : In order to determine the antibiotic resistance, the isolates were identified and assessed by the disk diffusion and agar dilution methods. This study investigated the gene expression of four multi-drug efflux pump systems (MexAB-OprM, MexCD-OprJ, MexEF-OprN and MexXY (-OprA)) and its correlation with antibiotic resistance.Results : The isolates indicated that the highest resistance rate was against ticarcillin (۸۰%), followed by ciprofloxacin (۷۴%) and meropenem (۷۱%). Most of them expressed mexB (۶۹%), mexC (۲۸.۷%), mexE (۴۳.۴%), and mexY (۷۴.۶%), suggesting that mexB and mexY were highly expressed in the studied strains. Conclusion : The overexpression of mexB and mexY was significantly more prevalent in the ICU wards (p=۰.۰۳۳). Furthermore, there was a significant correlation between the expression of RND-type efflux pumps and the resistance to most anti-pseudomonal antibiotics.
کلیدواژه ها:
نویسندگان
Aylin Esmailkhani
Department of Microbiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
Abed Zahedi bialvaei
Department of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran