Study of antimicrobial effect of Zataria multiflora, Thymus daenensis Celak, Alcea and Urtica on the growth of Escherichia coli in vitro

Background and Aim : The use of medicinal plants for treatment has a long history of human life. In recent years, the use of medicinal plants has increased due to the lower side effects and costs and patients' adaptation to these drugs and due to the existence of known side effects for chemical drugs.Methods : To evaluate the antibacterial effect of the plant extracts, susceptibility assay was performed by disk diffusion method. First, Escherichia coli was cultured uniformly on the Müller-Hinton agar medium. Then, sterile blank disks containing different dilutions of the extract (۱۰۰, ۵۰, ۲۵, ۱۲.۵ and ۶.۲۵) were placed on the agar medium. The culture media were then incubated for ۲۴ hours at ۳۷ ° C. Results : The results showed that the highest diameter of growth inhibition zone among different treatments is related to the use of fluorophenicol ۱۰%. In the next priority, Zataria multiflora and Thyme extract were concentrated in Escherichia coli culture medium at concentrations of ۱۰۰ and ۵۰%, respectively. . The diameter of the growth inhibition zone was related to the commercial drugs Enrofloxacin ۱۰% and sulfadiazine-trimethoprim (Soltrim ۴۸%), concentrations of ۲۵, ۱۲.۵% of thyme and Dena, and concentrations of ۱۰۰ and ۵۰% of nettle and marshmallow in one range and There was no significant difference with each other, but the diameter of the halo created by each of the treatments was significantly less than Zataria multiflora and Denai extracts in concentrations of ۱۰۰ and ۵۰% and also fluorophenicol (P <۰.۰۵).Conclusion : to compare the observed results on the antibacterial properties of different plant extracts, which can be explained by the differences in different laboratory methods of antibacterial properties of essential oils, method of preparation of essential oils, strain and type of plant and their sources. , Pointed out the growth stage of the plant as well as the bacterial strains used