Identification of oxa and ndm genes by Multiplex-PCR method and determination of drug resistance pattern of Acinetobacter baumannii isolates isolated from clinical samples

Background and Aim : Acinetobacter baumannii is the cause of a wide range of nosocomial infections. Antibiotic resistance of this organism is a major challenge worldwide. The aim of this study was to identify oxa and ndm genes by multiplex-PCR and to determine the pattern of drug resistance.Methods : This study was performed in a period of ۸ months by collecting ۲۵ bacterial isolates. Antibiotic susceptibility testing was performed on Müller Hinton agar medium by disk diffusion method. MPprimer software was used to design the primers. After data collection, the level of significance was assessed at P˂۰.۰۵ using SPSS ۲۲.Results : Except for gentamicin, cefpime, ciprofloxacin and tobramycin, there is a significant relationship between resistance pattern and sensitivity. All Acinetobacter baumannii isolates were sensitive to ceftazidime. Also, ۲۰% of the isolates were resistant to imipenem, which are considered carbapenem-resistant isolates of Acinetobacter baumannii. Of the ۲۵ isolates studied, ۵ isolates (۲۰%) had oxa۵۸ gene, ۵ isolates (۲۰%) had oxa۲۳ gene and only three isolates (۱۲%) had ndm gene.Conclusion : The results of this study showed a high widespread of antibiotic resistance among Acinetobacter baumannii isolates, which emphasizes the need to develop programs in the control and treatment of this powerful pathogen. Also, the frequency of beta-lactamase-producing isolates in hospital isolates has been growing, which indicates the need for more attention of health centers in prescribing drugs.

کلیدواژه ها:

Acinetobacter baumannii ، Antibiotic resistance ، Multiplex-PCR ، Nosocomial infection.