Detection of human papillomavirus (HPV) infections in Iranian women with and without abnormal cervical cytology by dot blot hybridization

Background and Aim : Infection with certain human papillomaviruses (HPV) is strongly associated with the development of dysplasia and cancer of the cervix uteri. The average global prevalence of HPV infection has been reported to be almost ۱.۴–۲۵.۶% in different geographical regains. To date, more than ۲۰۰ HPV genotypes have been identified, and more than forty of these have been shown to cause anogenital infections in humans. The aim of this study was to estimate the prevalence of HPV in ۵۴۰ cervical samples from patients who underwent annual routine gynecological exams by dot blot hybridization from different geographical regions in Iran.Methods : Cervical cells were collected from ۱۴ different city and were stored at ۴°C until DNA extraction. The extraction of total DNA was performed according to the manufacturer’s instruction (Add bio, Korea) and HPV genotyping by hybridization method (Operon, Spain). The data were recorded using Microsoft Excel ۲۰۰۷ (Microsoft Corp, Redmond, WA, USA) and analyzed with the SPSS software (version ۱۶; SPSS Inc., Chicago, IL, USA). P value < ۰.۰۵ was considered statistically significant. Results : Five hundred and forty women between ۱۸ and ۷۷ years (mean age: ۳۴ years) and ۲۱۷ (۴۰.۱۸ %) were HPV-DNA positive. A total of ۱۴۳ low-risk and ۱۴۳ high-risk HPV isolates were identified. HPV high-risk type ۳۱ showed the highest prevalence (۱۶.۵۸%), while in low risk samples, HPV type ۶ showed the highest prevalence ۳۰.۴۱%. Demographic data of positive HPV patients showed that ۳.۲۲%, ۴.۱۴%, ۴.۶۰%,۱.۳۸%, ۴.۶۰% and ۲.۳۰% with presence of raised lesions/discharge, abnormal bleeding, history use oral contraceptives pills, abnormal pap smear, menstrual irregularities and abortion, respectively. Conclusion : Due to the high prevalence of HPV is important for the selection of prevention strategies and improving approaches to combating cervical cancer. Our dot blot hybridization assay will be useful to address questions related to viral persistence at the genotype level, the kinetics of viral load and disease recurrence.Evidence is accumulating that HPV genotyping may be useful for patient management in the future.

کلیدواژه ها:

Hybridization ، Human papillomavirus ، cervical cancer