Enhanced Macrophage Activity against Methicillin-Resistant Staphylococcus aureus on Exposure to Glucomannan: An in-vitro study

Background and Aim : Methicillin-resistant Staphylococcus aureus (MRSA) causes a wide range of diseases and is one of the main causes of nosocomial infections. Carbohydrate glucomannan has adjuvant and immune regulating properties, which is an ideal candidate for immunological development. The aim of this study was to evaluate the level of macrophage gene expression and phagocytic activity against Methicillin-Resistant S. aureus in the presence of glucomannan. Methods : The effect of different concentrations of glucomannan were assessed on the phagocytic activity of macrophage cells by colony count method. The expression of tumor necrosis factor-alpha (TNF-α) and Inducible nitric oxide synthase (iNOS) genes were evaluated by Real-Time PCR.Results : The concentration of glucomannan significantly reduced the colony-forming units (CFUs) of MRSA and increased the phagocytosis of macrophages. The high level of iNOS and TNF gene expression was observed at ۱۰۰ g/mL concentration of glucomannan. Conclusion : The glucomannan may introduce as a stimulant of the immune system. It increases the expression of TNF-α and iNOS genes which are involved in the phagocytosis pathway of macrophages against MRSA.

کلیدواژه ها:

Glucomannan ، Macrophage Activity ، Methicillin-Resistant Staphylococcus aureus ، In-Vitro Study ، iNOS ، TNF-alpha